PUBLICATION

The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation

Authors
Morita, H., Grigolon, S., Bock, M., Krens, S.F., Salbreux, G., Heisenberg, C.P.
ID
ZDB-PUB-170222-18
Date
2017
Source
Developmental Cell   40(4): 354-366.e4 (Journal)
Registered Authors
Heisenberg, Carl-Philipp, Krens, S. F. Gabby, Morita, Hitoshi
Keywords
active fluid description of tissue spreading, gastrulation, interfacial tension, radial cell intercalation, surface cell expansion, zebrafish
MeSH Terms
  • Surface Tension
  • Gastrulation*
  • Cell Proliferation
  • Cell Count
  • Stress, Physiological
  • Cell Communication
  • Computer Simulation
  • Cell Movement
  • Blastoderm/cytology
  • Blastoderm/metabolism
  • Biophysical Phenomena*
  • Animals
  • Embryo, Nonmammalian/cytology
  • Morphogenesis*
  • Zebrafish/embryology*
  • Zebrafish/physiology*
(all 16)
PubMed
28216382 Full text @ Dev. Cell
Abstract
Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during "doming," when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction.
Genes / Markers
Marker Marker Type Name
chukGENEcomponent of inhibitor of nuclear factor kappa B kinase complex
myl12.1GENEmyosin, light chain 12, genome duplicate 1
1 - 2 of 2
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Figures
Figure Gallery (9 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e112TgTransgenic Insertion
    e114TgTransgenic Insertion
      e2212TgTransgenic Insertion
        p20ad
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          No data available
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-tjp1monoclonalIgG1Mouse
          Ab4-h3polyclonal
            		IgGRabbit
            1 - 2 of 2
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            1 - 2 of 2
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            Mapping
            No data available
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            Citation
            Morita, H., Grigolon, S., Bock, M., Krens, S.F., Salbreux, G., Heisenberg, C.P. (2017) The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation. Developmental Cell. 40(4):354-366.e4.