PUBLICATION

Zebrafish yap1 plays a role in differentiation of hair cells in posterior lateral line

Authors
Loh, S.L., Teh, C., Muller, J., Guccione, E., Hong, W., Korzh, V.
ID
ZDB-PUB-140513-403
Date
2014
Source
Scientific Reports   4: 4289 (Journal)
Registered Authors
Korzh, Vladimir, Loh, Siau Lin, Teh, Cathleen
Keywords
none
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Cell Differentiation/genetics*
  • Embryonic Development/genetics
  • Gene Expression
  • Signal Transduction
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Protein Binding
  • Hair Cells, Auditory/cytology*
  • Hair Cells, Auditory/metabolism*
  • Gene Knockdown Techniques
  • Animals
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
(all 15)
PubMed
24598795 Full text @ Sci. Rep.
Abstract
The evolutionarily conserved Hippo signaling pathway controls organ size by regulating cell proliferation and apoptosis and this process involves Yap1. The zebrafish Yap1 acts during neural differentiation, but its function is not fully understood. The detailed analysis of yap1 expression in proliferative regions, revealed it in the otic placode that gives rise to the lateral line system affected by the morpholino-mediated knockdown of Yap1. The comparative microarray analysis of transcriptome of Yap1-deficient embryos demonstrated changes in expression of many genes, including the Wnt signaling pathway and, in particular, prox1a known for its role in development of mechanoreceptors in the lateral line. The knockdown of Yap1 causes a deficiency of differentiation of mechanoreceptors, and this defect can be rescued by prox1a mRNA. Our studies revealed a role of Yap1 in regulation of Wnt signaling pathway and its target Prox1a during differentiation of mechanosensory cells.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
sqet4Et + MO4-tp53 + MO5-yap1standard conditionsLong-pec
sqet4Et + MO4-tp53 + MO5-yap1standard conditionsLong-pec
sqet4Et + MO4-tp53 + MO5-yap1standard conditionsProtruding-mouth
zf106Tg + MO4-tp53 + MO5-yap1standard conditionsPrim-25
1 - 4 of 4
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sqet4EtTransgenic Insertion
    sqet20EtTransgenic Insertion
      sqet33mi60AEtTransgenic Insertion
        zf106TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          kat6bMO1-kat6bMRPHLNO
          sin3abMO1-sin3abMRPHLNO
          tp53MO4-tp53MRPHLNO
          yap1MO4-yap1MRPHLNO
          yap1MO5-yap1MRPHLNO
          1 - 5 of 5
          Show
          Fish
          Fish
          sqet4Et
          sqet4Et + MO4-tp53 + MO5-yap1
          sqet20Et
          sqet20Et + MO4-tp53 + MO5-yap1
          sqet33mi60AEt
          sqet33mi60AEt + MO4-tp53 + MO5-yap1
          WT
          WT + MO4-tp53 + MO5-yap1
          zf106Tg
          zf106Tg + MO4-tp53 + MO5-yap1
          1 - 10 of 10
          Show
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
          Show
          Mapping
          No data available
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          Citation
          Loh, S.L., Teh, C., Muller, J., Guccione, E., Hong, W., Korzh, V. (2014) Zebrafish yap1 plays a role in differentiation of hair cells in posterior lateral line. Scientific Reports. 4:4289.