PUBLICATION

Core fucosylation is required for midline patterning during zebrafish development

Authors
Seth, A., Machingo, Q.J., Fritz, A., and Shur, B.D.
ID
ZDB-PUB-101115-20
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(12): 3380-3390 (Journal)
Registered Authors
Fritz, Andreas, Seth, Anandita
Keywords
zebrafish, fucosylation, apolipoprotein B, sonic hedgehog
MeSH Terms
  • Humans
  • Immunohistochemistry
  • Animals, Genetically Modified
  • Apolipoproteins B/genetics
  • Apolipoproteins B/metabolism
  • Blotting, Western
  • Reverse Transcriptase Polymerase Chain Reaction
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism
  • Fucosyltransferases/genetics
  • Fucosyltransferases/metabolism*
  • Zebrafish/embryology*
  • In Situ Hybridization
  • Body Patterning/genetics
  • Body Patterning/physiology*
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Hep G2 Cells
(all 19)
PubMed
21069830 Full text @ Dev. Dyn.
Abstract
Complex carbohydrates represent one of the most polymorphic classes of macromolecules, but their functions during embryonic development remain poorly defined. Herein, we show that knockdown of FucT8, the fucosyltransferase responsible for adding an α1,6 fucosyl residue to the core region of N-linked oligosaccharides, results in defective midline patterning during zebrafish development. Reduced FucT8 expression leads to mild cyclopia, small forebrains, U-shaped somites, among other midline patterning defects. One of the principal FucT8 substrates was identified as Apolipoprotein B (ApoB), the major scaffold protein that is responsible for assembly and secretion of lipoprotein particles in vertebrates. In Drosophila, lipoprotein particles are thought to facilitate cell signaling by serving as a transport vehicle for lipid-modified cell signaling proteins, such as hedgehog. In this regard, knockdown of ApoB expression in zebrafish embryos leads to similar midline patterning defects as those seen in FucT8 morphant embryos. Furthermore, preliminary studies suggest that ApoB facilitates Sonic hedgehog signaling during zebrafish development, analogous to the function of lipoprotein particles during hedgehog signaling in Drosophila.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
apobb.1MO1-apobb.1MRPHLNO
apobb.1MO2-apobb.1MRPHLNO
fut8aMO1-fut8aMRPHLNO
fut8aMO2-fut8aMRPHLNO
1 - 4 of 4
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Fish
Antibodies
Orthology
Gene Orthology
fut8a
1 - 1 of 1
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Engineered Foreign Genes
No data available
Mapping
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