PUBLICATION

The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish

Authors
Imai, F., Yoshizawa, A., Fujimori-Tonou, N., Kawakami, K., and Masai, I.
ID
ZDB-PUB-100826-2
Date
2010
Source
Development (Cambridge, England)   137(19): 3257-3268 (Journal)
Registered Authors
Imai, Fumiyasu, Kawakami, Koichi, Masai, Ichiro
Keywords
none
MeSH Terms
  • Cell Proliferation
  • Anaphase-Promoting Complex-Cyclosome
  • Cell Differentiation
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Ubiquitin-Protein Ligase Complexes/metabolism
  • Animals
  • Mice, Inbred C57BL
  • Lens, Crystalline/cytology*
  • Lens, Crystalline/embryology
  • Lens, Crystalline/metabolism*
  • Ubiquitin/metabolism*
  • Proteasome Endopeptidase Complex/metabolism*
  • Base Sequence
  • Mice
  • Epithelium/embryology
  • Epithelium/metabolism
(all 17)
PubMed
20724448 Full text @ Development
Abstract
In the developing vertebrate lens, epithelial cells differentiate into fiber cells, which are elongated and flat in shape and form a multilayered lens fiber core. In this study, we identified the zebrafish volvox (vov) mutant, which shows defects in lens fiber differentiation. In the vov mutant, lens epithelial cells fail to proliferate properly. Furthermore, differentiating lens fiber cells do not fully elongate, and the shape and position of lens fiber nuclei are affected. We found that the vov mutant gene encodes Psmd6, the subunit of the 26S proteasome. The proteasome regulates diverse cellular functions by degrading polyubiquitylated proteins. Polyubiquitylated proteins accumulate in the vov mutant. Furthermore, polyubiquitylation is active in nuclei of differentiating lens fiber cells, suggesting roles of the proteasome in lens fiber differentiation. We found that an E3 ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C) is involved in lens defects in the vov mutant. These data suggest that the ubiquitin proteasome system is required for cell proliferation of lens epithelium and for the differentiation of lens fiber cells in zebrafish.
Genes / Markers
Figures
Figure Gallery (16 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nk168aGtTransgenic Insertion
    nkuasgfp1aTgTransgenic Insertion
      rw0405bTgTransgenic Insertion
        rw0410hTgTransgenic Insertion
          rw619
            Point Mutation
            zdf1
              Point Mutation
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              fzr1aMO1-fzr1aMRPHLNO
              fzr1aMO2-fzr1aMRPHLNO
              psmc2MO1-psmc2MRPHLNO
              psmd6MO1-psmd6MRPHLNO
              1 - 4 of 4
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              Fish
              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              mAGFPEFGmAGFP
              mKOFP2EFGmKOFP2
              1 - 3 of 3
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              Mapping
              Entity Type Entity Symbol Location
              Featurerw619Chr: 11 Details
              GENEpsmd6Chr: 11 Details
              1 - 2 of 2
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