PUBLICATION

The zebrafish flotte lotte mutant reveals that the local retinal environment promotes the differentiation of proliferating precursors emerging from their stem cell niche

Authors
Cerveny, K.L., Cavodeassi, F., Turner, K.J., de Jong-Curtain, T.A., Heath, J.K., and Wilson, S.W.
ID
ZDB-PUB-100601-14
Date
2010
Source
Development (Cambridge, England)   137(13): 2107-2115 (Journal)
Registered Authors
Cavodeassi, Florencia, Cerveny, Kara, de Jong-Curtain, Tanya A., Heath, Joan K., Turner, Katherine, Wilson, Steve
Keywords
Ciliary marginal zone, Cell cycle progression, Differentiation, Neurogenesis, Retinal stem cells, Zebrafish
MeSH Terms
  • Feedback
  • Retina/cytology*
  • Retina/metabolism
  • Zebrafish/embryology*
  • Neurogenesis*
  • Organ Size
  • Stem Cells/cytology*
  • Apoptosis
  • Animals
  • Nuclear Pore Complex Proteins/genetics
  • Nuclear Pore Complex Proteins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 13)
PubMed
20504962 Full text @ Development
Abstract
It is currently unclear how intrinsic and extrinsic mechanisms cooperate to control the progression from self-renewing to neurogenic divisions in retinal precursor cells. Here, we use the zebrafish flotte lotte (flo) mutant, which carries a mutation in the elys (ahctf1) gene, to study the relationship between cell cycle progression and neuronal differentiation by investigating how proliferating progenitor cells transition towards differentiation in a retinal stem cell niche termed the ciliary marginal zone (CMZ). In zebrafish embryos without Elys, CMZ cells retain the capacity to proliferate but lose the ability to enter their final neurogenic divisions to differentiate as neurons. However, mosaic retinae composed of wild-type and flo cells show that despite inherent cell cycle defects, flo mutant cells progress from proliferation to differentiation when in the vicinity of wild-type retinal neurons. We propose that the differentiated retinal environment limits the proliferation of precursors emerging from the CMZ in a manner that explains the spatial organisation of cells in the CMZ and ensures that proliferative retinal progenitors are driven towards differentiation.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
knu3TgTransgenic Insertion
    t10TgTransgenic Insertion
      ti262c
        		Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        tp53MO4-tp53MRPHLNO
        1 - 1 of 1
        Show
        Fish
        Fish
        ahctf1ti262c/ti262c
        knu3Tg
        t10Tg
        1 - 3 of 3
        Show
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 2 of 2
        Show
        Mapping
        No data available
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        Citation
        Cerveny, K.L., Cavodeassi, F., Turner, K.J., de Jong-Curtain, T.A., Heath, J.K., and Wilson, S.W. (2010) The zebrafish flotte lotte mutant reveals that the local retinal environment promotes the differentiation of proliferating precursors emerging from their stem cell niche. Development (Cambridge, England). 137(13):2107-2115.