PUBLICATION

Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish

Authors
Veldman, M.B., Bemben, M.A., and Goldman, D.
ID
ZDB-PUB-100211-4
Date
2010
Source
Molecular and cellular neurosciences   43(4): 370-383 (Journal)
Registered Authors
Goldman, Dan, Veldman, Matt
Keywords
Retina, Retinal ganglion cell, Tubulin, KLF, Optic nerve, p27, Promoter
MeSH Terms
  • Electrophoretic Mobility Shift Assay
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • In Situ Hybridization
  • Animals, Genetically Modified
  • Promoter Regions, Genetic/genetics
  • Tubulin/genetics*
  • Tubulin/metabolism
  • Animals
  • Reverse Transcriptase Polymerase Chain Reaction
  • Optic Nerve Injuries/metabolism*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Nerve Regeneration/physiology*
  • Gene Expression Regulation
  • Immunohistochemistry
  • Zebrafish
  • Retina/metabolism*
(all 20)
PubMed
20123021 Full text @ Mol. Cell Neurosci.
Abstract
We report that knockdown of the alpha1 tubulin isoform Tuba1a, but not the highly related Tuba1b, dramatically impedes nervous system formation during development and RGC axon regeneration following optic nerve injury in adults. Within the tuba1a promoter, a G/C-rich element was identified that is necessary for tuba1a induction during RGC differentiation and optic axon regeneration. KLF6a and 7a, which we previously reported are essential for optic axon regeneration (Veldman et al., 2007), bind this G/C-rich element and transactivate the tuba1a promoter. In vivo knockdown of KLF6a and 7a attenuate regeneration-dependent activation of the endogenous tuba1a and p27 genes. These results suggest tuba1a expression is necessary for CNS development and regeneration and that KLF6a and 7a mediate their effects, at least in part, via transcriptional control of tuba1a promoter activity.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-tuba1astandard conditionsPrim-15
WT + MO1-tuba1astandard conditionsPrim-15
WT + MO1-tuba1astandard conditionsPrim-15
WT + MO1-tuba1astandard conditionsPrim-15
WT + MO1-tuba1astandard conditionsPrim-15
WT + MO1-tuba1astandard conditionsPrim-15
WT + MO1-tuba1astandard conditionsPrim-25
WT + MO1-tuba1astandard conditionsPrim-25
WT + MO1-tuba1bstandard conditionsPrim-15
WT + MO2-tuba1astandard conditionsPrim-15
1 - 10 of 18
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mi2TgTransgenic Insertion
    mi22TgTransgenic Insertion
      mi23TgTransgenic Insertion
        mi24TgTransgenic Insertion
          mi29TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            klf6aMO1-klf6aMRPHLNO
            klf6aMO2-klf6aMRPHLNO
            klf7bMO1-klf7bMRPHLNO
            klf7bMO2-klf7bMRPHLNO
            tuba1aMO1-tuba1aMRPHLNO
            tuba1aMO2-tuba1aMRPHLNO
            tuba1bMO1-tuba1bMRPHLNO
            tuba1bMO2-tuba1bMRPHLNO
            1 - 8 of 8
            Show
            Fish
            Fish
            mi2Tg
            mi2Tg + MO1-klf6a + MO1-klf7b + MO2-klf6a + MO2-klf7b
            mi22Tg
            mi23Tg
            mi24Tg
            mi29Tg
            WT
            WT + MO1-tuba1a
            WT + MO1-tuba1b
            WT + MO2-tuba1a
            1 - 10 of 11
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            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            1 - 2 of 2
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            Mapping
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            Citation
            Veldman, M.B., Bemben, M.A., and Goldman, D. (2010) Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish. Molecular and cellular neurosciences. 43(4):370-383.