PUBLICATION

Basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility

Authors

Lang, M.R., Patterson, L.B., Gordon, T.N., Johnson, S.L., and Parichy, D.M.

ID

ZDB-PUB-091215-35

Date

2009

Source

PLoS Genetics 5(11): e1000744 (Journal)

Registered Authors

Johnson, Stephen L., Lang, Michael, Parichy, David M., Patterson, Larissa

Keywords

Melanophores, Pigments, Chromatophores, Zebrafish, Pattern formation, Embryonic pattern formation, Epidermis, Phenotypes

MeSH Terms

Animals
Mutation
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Ovary/pathology
Fertility*
Zinc Fingers
Melanophores
Body Patterning
Pigmentation*
Carrier Proteins/genetics
Carrier Proteins/physiology*
Female
Zebrafish

(all 14)

PubMed

19956727 Full text @ PLoS Genet.

Abstract

Relatively little is known about the generation of adult form. One complex adult trait that is particularly amenable to genetic and experimental analysis is the zebrafish pigment pattern, which undergoes extensive remodeling during post-embryonic development to form adult stripes. These stripes result from the arrangement of three classes of neural crest-derived pigment cells, or chromatophores: melanophores, xanthophores, and iridophores. Here, we analyze the zebrafish bonaparte mutant, which has a normal early pigment pattern but exhibits a severe disruption to the adult stripe pattern. We show that the bonaparte mutant phenotype arises from mutations in basonuclin-2 (bnc2), encoding a highly conserved, nuclear-localized zinc finger protein of unknown function. We show that bnc2 acts non-autonomously to the melanophore lineage and is expressed by hypodermal cells adjacent to chromatophores during adult pigment pattern formation. In bonaparte (bnc2) mutants, all three types of chromatophores differentiate but then are lost by extrusion through the skin. We further show that while bnc2 promotes the development of two genetically distinct populations of melanophores in the body stripes, chromatophores of the fins and scales remain unaffected in bonaparte mutants, though a requirement of fin chromatophores for bnc2 is revealed in the absence of kit and colony stimulating factor-1 receptor activity. Finally, we find that bonaparte (bnc2) mutants exhibit dysmorphic ovaries correlating with infertility and bnc2 is expressed in somatic ovarian cells, whereas the related gene, bnc1, is expressed within oocytes; and we find that both bnc2 and bnc1 are expressed abundantly within the central nervous system. These findings identify bnc2 as an important mediator of adult pigment pattern formation and identify bonaparte mutants as an animal model for dissecting bnc2 functions.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
bnc1	WT	standard conditions	Long-pec		brain	ISH	Fig. 5
bnc1	WT	standard conditions	Long-pec		cranial ganglion	ISH	Fig. 5
bnc1	WT	standard conditions	14-19 somites		ectoderm	ISH	Fig. 5
bnc1	WT	standard conditions	Prim-5		head	ISH	Fig. 5
bnc1	WT	standard conditions	Days 14-20	Not Detected	hypodermal cell	ISH	Fig. 6
bnc1	WT	standard conditions	Adult		integument	RTPCR	text only
bnc1	WT	standard conditions	Days 14-20	Not Detected	integument	ISH	Fig. 6
bnc1	WT	standard conditions	Adult		kidney	RTPCR	text only
bnc1	WT	standard conditions	14-19 somites		mesoderm	ISH	Fig. 5
bnc1	WT	standard conditions	Prim-5		myotome	ISH	Fig. 5

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
bnc2^j10e1/j10e1	standard conditions	Adult	anal fin melanophore stripe present, normal	Fig. 1
bnc2^j10e1/j10e1	standard conditions	Adult	caudal fin melanophore stripe present, normal	Fig. 1
bnc2^j10e1/j10e1	standard conditions	Adult	pigmentation disrupted, abnormal	Fig. 1
bnc2^j10e1/j10e1	standard conditions	Adult	trunk melanophore stripe disorganized, abnormal	Fig. 1
bnc2^j10e1/j10e1	standard conditions	Adult	whole organism female sterile, abnormal	Fig. 1
bnc2^{utr16e1/utr16e1}	standard conditions	Days 14-20 to Days 21-29	pigment cell (sensu Vertebrata) decreased amount, abnormal	Fig. 8
bnc2^{utr16e1/utr16e1}	standard conditions	Days 14-20 to Days 21-29	pigment cell development disrupted, abnormal	Fig. 8
bnc2^{utr16e1/utr16e1}	standard conditions	Days 14-20 to Adult	anal fin melanophore stripe present, normal	Fig. 1
bnc2^{utr16e1/utr16e1}	standard conditions	Days 14-20 to Adult	caudal fin melanophore stripe present, normal	Fig. 1
bnc2^{utr16e1/utr16e1}	standard conditions	Days 14-20 to Adult	hypodermis iridophore decreased amount, abnormal	Fig. 1

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b5	Small Deletion	kita
b140	Unknown	ednrba
j4blue	Small Deletion	csf1ra
j9s1	Point Mutation	ltk
j10e1	Point Mutation	bnc2
utr16e1	Point Mutation	bnc2
w2	Point Mutation	mitfa

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
bnc2^j10e1/j10e1
bnc2^{utr16e1/utr16e1}
bnc2^{utr16e1/utr16e1}; csf1ra^{j4blue/j4blue}
bnc2^{utr16e1/utr16e1}; csf1ra^{j4blue/j4blue}; kita^b5/b5
bnc2^{utr16e1/utr16e1}; kita^b5/b5
bnc2^{utr16e1/utr16e1}; ltk^j9s1/j9s1
csf1ra^{j4blue/j4blue}
csf1ra^{j4blue/j4blue}; ednrba^b140/b140
csf1ra^{j4blue/j4blue}; kita^b5/b5
kita^b5/b5

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Antibodies

Orthology

Gene	Orthology
bnc1

Engineered Foreign Genes

Mapping

Lang et al., 2009 ZDB-PUB-091215-35 PMID:19956727

Basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility

Lang et al., 2009

ZDB-PUB-091215-35
PMID:19956727