PUBLICATION

Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta

Authors
Wilkinson, R.N., Pouget, C., Gering, M., Russell, A.J., Davies, S.G., Kimelman, D., and Patient, R.
ID
ZDB-PUB-090622-7
Date
2009
Source
Developmental Cell   16(6): 909-916 (Journal)
Registered Authors
Gering, Martin, Kimelman, David, Patient, Roger K., Wilkinson, Robert
Keywords
DEVBIO, STEMCELL
MeSH Terms
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism
  • Bone Morphogenetic Protein 4/metabolism*
  • Arteries/cytology
  • Arteries/metabolism
  • Hedgehog Proteins/metabolism*
  • Body Patterning*
  • Cell Polarity*
  • Signal Transduction
  • Gene Expression Regulation, Developmental
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Aorta/cytology*
  • Aorta/embryology
  • Zebrafish/embryology*
  • Core Binding Factor Alpha 2 Subunit/metabolism
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
(all 21)
PubMed
19531361 Full text @ Dev. Cell
Abstract
Hematopoietic stem cells (HSCs) are first detected in the floor of the embryonic dorsal aorta (DA), and we investigate the signals that induce the HSC program there. We show that while continued Hedgehog (Hh) signaling from the overlying midline structures maintains the arterial program characteristic of the DA roof, a ventral Bmp4 signal induces the blood stem cell program in the DA floor. This patterning of the DA by Hh and Bmp is the mirror image of that in the neural tube, with Hh favoring dorsal rather than ventral cell types, and Bmp favoring ventral rather than dorsal. With the majority of current data supporting a model whereby HSCs derive from arterial endothelium, our data identify the signal driving this conversion. These findings are important for the study of the production of HSCs from embryonic stem cells and establish a paradigm for the development of adult stem cells.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fr13TgTransgenic Insertion
    tt250
      		Point Mutation
      w30TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        bmp4MO3-bmp4MRPHLNO
        1 - 1 of 1
        Show
        Fish
        Fish
        chrdtt250/tt250
        fr13Tg/+
        w30Tg/+
        1 - 3 of 3
        Show
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
        Show
        Mapping
        No data available
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        Citation
        Wilkinson, R.N., Pouget, C., Gering, M., Russell, A.J., Davies, S.G., Kimelman, D., and Patient, R. (2009) Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta. Developmental Cell. 16(6):909-916.