PUBLICATION

Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain

Authors
Ohata, S., Kinoshita, S., Aoki, R., Tanaka, H., Wada, H., Tsuruoka-Kinoshita, S., Tsuboi, T., Watabe, S., and Okamoto, H.
ID
ZDB-PUB-090417-11
Date
2009
Source
Development (Cambridge, England)   136(10): 1653-1663 (Journal)
Registered Authors
Ohata, Shinya, Okamoto, Hitoshi, Tanaka, Hideomi, Wada, Hironori
Keywords
none
MeSH Terms
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics
  • Stem Cells/physiology*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/physiology
  • Polysaccharides/physiology*
  • Vagus Nerve/cytology
  • Vagus Nerve/embryology
  • Vagus Nerve/physiology*
  • Molecular Sequence Data
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology*
  • Cell Movement
  • Body Patterning
  • Animals
  • Motor Neurons/physiology*
  • Animals, Genetically Modified
  • Mutation
  • Hydro-Lyases/genetics
  • Amino Acid Sequence
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Neuroepithelial Cells/physiology*
(all 23)
PubMed
19369395 Full text @ Development
Abstract
The molecular mechanisms by which neurons migrate and accumulate to form the neural layers and nuclei remain unclear. The formation of vagus motor nuclei in zebrafish embryos is an ideal model system in which to address this issue because of the transparency of the embryos and the availability of established genetic and molecular biological techniques. To determine the genes required for the formation of the vagus motor nuclei, we performed N-ethyl-N-nitrosourea-based mutant screening using a zebrafish line that expresses green fluorescent protein in the motor neurons. In wild-type embryos, the vagus motor neuron progenitors are born in the ventral ventricular zone, then migrate tangentially in the dorsolateral direction, forming the nuclei. However, in towhead (twd(rw685)) mutant embryos, the vagus motor neuron progenitors stray medially away from the normal migratory pathway and fail to stop in the right location. The twd(rw685) mutant has a defect in the GDP-mannose 4,6 dehydratase (gmds) gene, which encodes a key enzyme in the fucosylation pathway. Levels of fucosylated glycans were markedly and specifically reduced in twd(rw685) mutant embryos. Cell transplantation analysis revealed that GMDS is not essential in the vagus motor neuron progenitors for correct formation of the vagus motor nuclei, but is required in the neuroepithelial cells that surround the progenitors. Together, these findings suggest that fucosylated glycans expressed in neuroepithelial cells are required to guide the migration of vagus motor neuron progenitors.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
rw0TgTransgenic Insertion
    rw685
      		Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Fish
      Antibodies
      Orthology
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
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      Citation
      Ohata, S., Kinoshita, S., Aoki, R., Tanaka, H., Wada, H., Tsuruoka-Kinoshita, S., Tsuboi, T., Watabe, S., and Okamoto, H. (2009) Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain. Development (Cambridge, England). 136(10):1653-1663.