PUBLICATION

EphA4 and EfnB2a maintain rhombomere coherence by independently regulating intercalation of progenitor cells in the zebrafish neural keel

Authors
Kemp, H.A., Cooke, J.E., and Moens, C.B.
ID
ZDB-PUB-090116-13
Date
2009
Source
Developmental Biology   327(2): 313-326 (Journal)
Registered Authors
Cooke, Julie E., Kemp, Hilary, Moens, Cecilia
Keywords
Eph, Ephrin, Efn, hindbrain, boundary, zebrafish, rhombomere, neuroepithelium, cell affinity, cell sorting
MeSH Terms
  • Male
  • Morphogenesis/physiology*
  • Stem Cells/cytology
  • Stem Cells/physiology*
  • Mosaicism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Cell Differentiation/physiology
  • Ephrin-B2/genetics
  • Ephrin-B2/metabolism*
  • Humans
  • Cell Adhesion/physiology
  • Female
  • Receptor, EphA4/genetics
  • Receptor, EphA4/metabolism*
  • Rhombencephalon*/cytology
  • Rhombencephalon*/embryology
  • Animals
  • Cell Division/physiology
  • Spindle Apparatus/metabolism
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
(all 22)
PubMed
19135438 Full text @ Dev. Biol.
Abstract
During vertebrate development, the hindbrain is transiently segmented into 7 distinct rhombomeres (r). Hindbrain segmentation takes place within the context of the complex morphogenesis required for neurulation, which in zebrafish involves a characteristic cross-midline division that distributes progenitor cells bilaterally in the forming neural tube. The Eph receptor tyrosine kinase EphA4 and the membrane-bound Ephrin (Efn) ligand EfnB2a, which are expressed in complementary segments in the early hindbrain, are required for rhombomere boundary formation. We showed previously that EphA4 promotes cell-cell affinity within r3 and r5, and proposed that preferential adhesion within rhombomeres contributes to boundary formation. Here we show that EfnB2a is similarly required in r4 for normal cell affinity and that EphA4 and EfnB2a regulate cell affinity independently within their respective rhombomeres. Live imaging of cell sorting in mosaic embryos shows that both proteins function during cross-midline cell divisions in the hindbrain neural keel. Consistent with this, mosaic EfnB2a over-expression causes widespread cell sorting and disrupts hindbrain organization, but only if induced at or before neural keel stage. We propose a model in which Eph and Efn-dependent cell affinity within rhombomeres serve to maintain rhombomere organization during the potentially disruptive process of teleost neurulation.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e1TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    efnb2aMO1-efnb2aMRPHLNO
    efnb2aMO2-efnb2aMRPHLNO
    epha4aMO1-epha4aMRPHLNO
    1 - 3 of 3
    Show
    Fish
    1 - 3 of 3
    Show
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab1-efnb2polyclonalIgGGoat
    Ab1-lampolyclonal
      IgGRabbit
      Ab1-prkczpolyclonalIgGRabbit
      Ab-3A10monoclonal
        IgG1Mouse
        1 - 4 of 4
        Show
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
        Show
        Mapping
        No data available