PUBLICATION

Six1a is required for the onset of fast muscle differentiation in zebrafish

Authors
Bessarab, D.A., Chong, S.W., Srinivas, B.P., and Korzh, V.
ID
ZDB-PUB-080922-1
Date
2008
Source
Developmental Biology   323(2): 216-228 (Journal)
Registered Authors
Bessarab, Dmitry, Chong, Shang Wei, Korzh, Vladimir, Srinivas, B. P.
Keywords
Six1, six1a, six1b, myogenin, fast muscle differentiation
MeSH Terms
  • Cell Differentiation
  • Organogenesis*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Phenotype
  • Somites/cytology
  • Somites/embryology
  • Organ Specificity
  • Cell Proliferation
  • Gene Expression Regulation, Developmental
  • Stem Cells/cytology
  • Zebrafish/embryology*
  • Muscle Fibers, Fast-Twitch/cytology
  • Muscle Fibers, Fast-Twitch/metabolism
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Muscles/embryology*
  • Sequence Homology, Amino Acid
(all 23)
PubMed
18789916 Full text @ Dev. Biol.
Abstract
Vertebrate skeletal muscles arise from two major types of precursor cell populations which differentiate into slow and fast fibers. Six1 homeodomain transcription factor was implicated in myogenesis in mammals, but its role in the development of different types of muscle precursors remained unclear. In zebrafish, there are two close homologs of Six1: six1a (known earlier as six1) and six1b identified in this study. Here we studied the role of six1a whose expression is initiated in the fast muscle precursor region of the forming somite. In the six1a loss-of-function conditions, initiation of myog expression was compromised in fast muscle precursors whereas myod expression appeared unaffected suggestive of six1a requirement for fast muscle differentiation. Expression of myog recovered soon, but differentiation of fast muscle proceeded abnormally. Exclusion of muscle-specific transcripts, myhz1 and tpma, from the dorsal and posterior part of somites demonstrated early abnormalities in fast muscle formation. U-shaped somites, reduced birefringence, and abnormal cell morphology were observed in morphant fast muscle upon terminal differentiation. In contrast, differentiation of slow fibers appeared largely unaffected. We conclude that Six1a plays an essential role at the onset of fast muscle differentiation.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB + MO4-six1bstandard conditions5-9 somites
AB + MO4-six1bstandard conditions5-9 somites
AB + MO4-six1bstandard conditions5-9 somites
AB + MO4-six1bstandard conditions5-9 somites
AB + MO4-six1bstandard conditions5-9 somites
AB + MO4-six1bstandard conditions10-13 somites
AB + MO4-six1bstandard conditions10-13 somites
AB + MO4-six1bstandard conditions10-13 somites
AB + MO4-six1bstandard conditionsPrim-5
AB + MO4-six1bstandard conditionsPrim-5
1 - 10 of 28
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gz5TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    six1bMO4-six1bMRPHLNO
    six1bMO5-six1bMRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    AB
    AB + MO4-six1b
    AB + MO5-six1b
    gz5Tg
    gz5Tg + MO4-six1b
    1 - 5 of 5
    Show
    Antibodies
    Orthology
    Gene Orthology
    six1a
    six2a
    1 - 2 of 2
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    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Bessarab, D.A., Chong, S.W., Srinivas, B.P., and Korzh, V. (2008) Six1a is required for the onset of fast muscle differentiation in zebrafish. Developmental Biology. 323(2):216-228.