PUBLICATION

Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation

Authors
Martin, B.L., and Kimelman, D.
ID
ZDB-PUB-080714-9
Date
2008
Source
Developmental Cell   15(1): 121-133 (Journal)
Registered Authors
Kimelman, David, Martin, Benjamin
Keywords
none
MeSH Terms
  • Protein Structure, Tertiary
  • Gene Expression Regulation, Developmental
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Oligonucleotides, Antisense/pharmacology
  • Somites/embryology
  • Models, Biological
  • Fetal Proteins
  • Body Patterning/physiology
  • Animals
  • Immunohistochemistry
  • Signal Transduction*
  • MyoD Protein/metabolism
  • Embryo, Nonmammalian/physiology
  • T-Box Domain Proteins/chemistry
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/physiology*
  • Mutation
  • Mesoderm/physiology*
  • Wnt Proteins/physiology*
  • In Situ Hybridization
  • Microinjections
  • Zebrafish/embryology*
  • Zebrafish/genetics
(all 25)
PubMed
18606146 Full text @ Dev. Cell
Abstract
The T box transcription factor Brachyury is essential for the formation of the posterior body in all vertebrates, although its critical transcriptional targets have remained elusive. Loss-of-function studies of mouse Brachyury and the zebrafish Brachyury ortholog Ntl indicated that Brachyury plays a more significant role in higher vertebrates than lower vertebrates. We have identified a second zebrafish Brachyury ortholog (Bra), and show that a combined loss of Ntl and Bra recapitulates the mouse phenotype, demonstrating an ancient role for Brachyury in patterning all but the most anterior somites. Using cell transplantation, we show that the only essential role for Brachyury during somite formation is non-cell autonomous, and demonstrate that Ntl and Bra are required for and can induce expression of the canonical Wnts wnt8 and wnt3a. We propose that a positive autoregulatory loop between Ntl/Bra and canonical Wnt signaling maintains the mesodermal progenitors to facilitate posterior somite development in chordates.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b195
    Insertion
    w26TgTransgenic Insertion
      w32TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        tbxtaMO3-tbxtaMRPHLNO
        tbxtbMO1-tbxtbMRPHLNO
        tbxtbMO2-tbxtbMRPHLNO
        tp53MO4-tp53MRPHLNO
        1 - 4 of 4
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        Fish
        Antibodies
        No data available
        Orthology
        Gene Orthology
        tbxtb
        1 - 1 of 1
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        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
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        Mapping
        No data available