PUBLICATION

Tensile forces govern germ-layer organization in zebrafish

Authors
Krieg, M., Arboleda-Estudillo, Y., Puech, P.H., Käfer, J., Graner, F., Müller, D.J., and Heisenberg, C.P.
ID
ZDB-PUB-080331-2
Date
2008
Source
Nature cell biology   10(4): 429-436 (Journal)
Registered Authors
Heisenberg, Carl-Philipp
Keywords
none
MeSH Terms
  • Stress, Mechanical
  • Signal Transduction/physiology
  • Body Patterning/physiology*
  • Nodal Protein
  • Animals
  • Microscopy, Atomic Force
  • Cytoskeleton/metabolism*
  • Cytoskeleton/ultrastructure
  • Cell Adhesion/physiology*
  • Germ Layers*/physiology
  • Germ Layers*/ultrastructure
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • Cell Aggregation/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Zebrafish/embryology*
  • Stem Cells/cytology
  • Stem Cells/physiology
(all 21)
PubMed
18364700 Full text @ Nat. Cell Biol.
Abstract
Understanding the factors that direct tissue organization during development is one of the most fundamental goals in developmental biology. Various hypotheses explain cell sorting and tissue organization on the basis of the adhesive and mechanical properties of the constituent cells. However, validating these hypotheses has been difficult due to the lack of appropriate tools to measure these parameters. Here we use atomic force microscopy (AFM) to quantify the adhesive and mechanical properties of individual ectoderm, mesoderm and endoderm progenitor cells from gastrulating zebrafish embryos. Combining these data with tissue self-assembly in vitro and the sorting behaviour of progenitors in vivo, we have shown that differential actomyosin-dependent cell-cortex tension, regulated by Nodal/TGFbeta-signalling (transforming growth factor beta), constitutes a key factor that directs progenitor-cell sorting. These results demonstrate a previously unrecognized role for Nodal-controlled cell-cortex tension in germ-layer organization during gastrulation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tz257
    Point Mutation
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Fish
    1 - 3 of 3
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    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    AB1-cdh1polyclonalRabbit
    Ab1-pan-CadherinpolyclonalRabbit
    1 - 2 of 2
    Show
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available