PUBLICATION

Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud

Authors

Lee, B.C., and Roy, S.

ID

ZDB-PUB-060906-22

Date

2006

Source

Developmental Biology 300(2): 623-634 (Journal)

Registered Authors

Lee, Ban Chuan, Roy, Sudipto

Keywords

Blimp-1, Limb bud, Zebrafish, Pectoral fin, tbx5, fgf24, fgf10, Apical ectodermal ridge, Zone of polarizing activity, Sonic hedgehog

MeSH Terms

Zebrafish Proteins/biosynthesis
Zebrafish Proteins/genetics*
Extremities/embryology*
Ectoderm/metabolism
Ectoderm/physiology
DNA-Binding Proteins/biosynthesis
DNA-Binding Proteins/genetics*
Limb Buds
Animals
Nuclear Proteins/biosynthesis
Nuclear Proteins/genetics*
Zebrafish/embryology*
Zebrafish/genetics*
Zebrafish/metabolism
Gene Expression Regulation, Developmental/physiology*

(all 15)

PubMed

16949569 Full text @ Dev. Biol.

Abstract

Formation of paired limbs in vertebrate embryos has long been a particularly useful paradigm for the study of pattern formation. Here, we show that Blimp-1, a SET domain and zinc finger-containing transcriptional factor, plays an important role in the development of the pectoral fins of the zebrafish structures that are homologous to forelimbs of amniotes. The blimp-1 gene is expressed dynamically in the mesenchyme as well as the ectodermal cells of the early fin bud, and later, in the cells of the apical ectodermal ridge (AER) of the outgrowing fin. Consistent with this expression profile, loss of Blimp-1 activity severely impairs fin outgrowth and patterning. We present evidence that blimp-1 functions downstream of tbx5 and fgf24 and therefore is not required for the initial specification of the fin bud primordia. Subsequently, however, its function is necessary for the induction of fgf10 and sonic hedgehog in the mesenchyme. In addition, Blimp-1 activity is absolutely critical for the proper induction of gene expression in the ectoderm and establishment of the AER. Taken together, these results identify an additional layer of control in the genetic pathway that operates in the developing limb and provides novel insights into regulatory mechanisms that organize its pattern.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dlx2a	prdm1a^tp39	standard conditions	Prim-15 to Prim-25		apical ectodermal ridge pectoral fin bud	ISH	Fig. 8
dlx2a	WT	standard conditions	Prim-15		apical ectodermal ridge pectoral fin bud	ISH	Fig. 2
dlx2a	WT	standard conditions	Prim-15 to Prim-25		apical ectodermal ridge pectoral fin bud	ISH	Fig. 8
dlx2a	WT + MO2-prdm1a	standard conditions	Prim-15 to Prim-25	Not Detected	apical ectodermal ridge pectoral fin bud	ISH	Fig. 8
fgf10a	prdm1a^tp39	standard conditions	Prim-5		mesenchyme	ISH	Fig. 6
fgf10a	prdm1a^tp39	standard conditions	Prim-25		mesenchyme pectoral fin	ISH	Fig. 6
fgf10a	prdm1a^tp39	standard conditions	Prim-15		mesenchyme pectoral fin	ISH	Fig. 6
fgf10a	prdm1a^tp39	standard conditions	Prim-5		pectoral fin bud	ISH	Fig. 6
fgf10a	WT	standard conditions	Prim-5		mesenchyme	ISH	Fig. 6
fgf10a	WT	standard conditions	Prim-25		mesenchyme pectoral fin	ISH	Fig. 6

1 - 10 of 61

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO2-prdm1a	standard conditions	Protruding-mouth	cleithrum decreased size, abnormal	Fig. 3
WT + MO2-prdm1a	standard conditions	Protruding-mouth	pectoral fin bud hypoplastic, abnormal	Fig. 3
WT + MO2-prdm1a	standard conditions	Protruding-mouth	pectoral fin skeleton aplastic, abnormal	Fig. 3
prdm1a^tp39	standard conditions	Protruding-mouth	pectoral fin skeleton decreased size, abnormal	Fig. 3
prdm1a^tp39	standard conditions	Protruding-mouth	pectoral fin truncated, abnormal	Fig. 3

1 - 5 of 5

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b641	Point Mutation	smo
t4	Deficiency	shha
tp39	Point Mutation	prdm1a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
fgf24	MO2-fgf24	MRPHLNO
prdm1a	MO2-prdm1a	MRPHLNO
tbx5a	MO1-tbx5a	MRPHLNO

Fish

Fish
Df(Chr07)t4/t4
prdm1a^tp39
smo^b641
WT
WT + MO1-tbx5a
WT + MO2-fgf24
WT + MO2-prdm1a

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Lee et al., 2006 ZDB-PUB-060906-22 PMID:16949569

Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud

Lee et al., 2006

ZDB-PUB-060906-22
PMID:16949569