PUBLICATION

Zebrafish foxi one modulates cellular responses to Fgf signaling required for the integrity of ear and jaw patterning

Authors

Nissen, R.M., Yan, J., Amsterdam, A., Hopkins, N., and Burgess, S.M.

ID

ZDB-PUB-030425-24

Date

2003

Source

Development (Cambridge, England) 130(11): 2543-2554 (Journal)

Registered Authors

Amsterdam, Adam, Burgess, Shawn, Hopkins, Nancy, Nissen, Robert M., Yan, Jizhou

Keywords

none

MeSH Terms

Base Sequence
Signal Transduction
Body Patterning/genetics
Mutation
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Neural Crest/cytology
Trans-Activators/genetics
Trans-Activators/metabolism
Branchial Region/embryology
Animals
DNA, Complementary/genetics
Models, Biological
Fibroblast Growth Factors/metabolism*
Nuclear Proteins*
Zebrafish/embryology*
Zebrafish/genetics*
Zebrafish/metabolism
Fibroblast Growth Factor 3
Jaw/embryology
Proto-Oncogene Proteins/metabolism
Forkhead Transcription Factors
DNA-Binding Proteins/genetics*
DNA-Binding Proteins/metabolism
Jaw Abnormalities/embryology
Jaw Abnormalities/genetics
Gene Expression Regulation, Developmental
Cell Movement
Paired Box Transcription Factors
Alleles
Ear/abnormalities
Ear/embryology
Biological Evolution
Transcription Factors/genetics*
Transcription Factors/metabolism

(all 35)

PubMed

12702667 Full text @ Development

Abstract

We identified four insertional alleles of foxi one (foo), an embryonic lethal mutation in zebrafish that displays defects in both otic placode and the jaw. In foo/foo embryos the otic placode is split into two smaller placodes and mutant embryos show a dorsoventral (DV) cartilage defect manifested as a reduced hyomandibular and reduced third and fourth branchial arches. We identified foxi one (foo), the zebrafish ortholog of Foxi1 (FREAC6, FKHL10, HFH-3, Fkh10) and a member of the forkhead domain transcriptional regulator family, as the gene mutated in foo/foo embryos. foo is expressed in otic placode precursor cells, and foo/foo embryos lack placodal pax8 expression and have disorganized otic expression of pax2.1 and dlx3. Third stream neural crest cell migration , detected by dlx2 and krox20 expression, is aberrant in that it invades the otic placode territory. foo is expressed in pharyngeal pouch endoderm and is required for pouch expression of pax8 and proper patterning of other markers in the pouch such as nkx2.3. In foo/foo embryos, we observed a failure to maintain fgf3 expression in the pouches, followed by apoptosis of neural crest cells in adjacent arches We conclude that foo expression is essential for pax8 expression probably downstream of Fgf signaling in a conserved pathway jointly required for integrity of patterning in the otic placode and pharyngeal pouches. We propose that correct placement of survival/proliferation cues is essential for shaping the pharyngeal cartilages and that evolutionary links between jaw and ear formation can be traced to Fgf- Foxi1-Pax8 pathways.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dlx2a	foxi1^hi3747Tg	standard conditions	Prim-5		brain	ISH	Fig. 7
dlx2a	foxi1^hi3747Tg	standard conditions	10-13 somites		neural crest hindbrain	ISH	Fig. 4
dlx2a	foxi1^hi3747Tg	standard conditions	Prim-5	Not Detected	pharyngeal arch 3 neural crest	ISH	Fig. 7
dlx2a	foxi1^hi3747Tg	standard conditions	Prim-5	Not Detected	pharyngeal arch 4 neural crest	ISH	Fig. 7
dlx2a	WT	standard conditions	Prim-5		brain	ISH	Fig. 7
dlx2a	WT	standard conditions	10-13 somites		neural crest hindbrain	ISH	Fig. 4
dlx2a	WT	standard conditions	Prim-5		pharyngeal arch 3 neural crest	ISH	Fig. 7
dlx2a	WT	standard conditions	Prim-5		pharyngeal arch 4 neural crest	ISH	Fig. 7
dlx3b	foxi1^hi3747Tg	standard conditions	10-13 somites		otic placode	ISH	Fig. 3
dlx3b	foxi1^hi3747Tg	standard conditions	1-4 somites		otic placode	ISH	Fig. 3

1 - 10 of 78

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-fgf3	standard conditions	Prim-5	hyosymplectic cartilage hypoplastic, abnormal	Fig. 8
foxi1^hi3747Tg	standard conditions	10-13 somites	neural crest cell migration process quality, abnormal	Fig. 4
foxi1^hi3747Tg	standard conditions	14-19 somites	otic vesicle decreased size, abnormal	Fig. 1
foxi1^hi3747Tg	standard conditions	14-19 somites	otic vesicle development process quality, abnormal	Fig. 1
foxi1^hi3747Tg	standard conditions	14-19 somites	otic vesicle split, abnormal	Fig. 1
foxi1^hi3747Tg	standard conditions	Prim-5	otic vesicle decreased size, abnormal	Fig. 1
foxi1^hi3747Tg	standard conditions	Prim-5	otic vesicle development process quality, abnormal	Fig. 1
foxi1^hi3747Tg	standard conditions	Prim-5	otic vesicle split, abnormal	Fig. 1
foxi1^hi3747Tg	standard conditions	Prim-5	pharyngeal pouch 1 apoptotic process increased process quality, abnormal	Fig. 8
foxi1^hi3747Tg	standard conditions	Prim-5	pharyngeal pouch 2 apoptotic process increased process quality, abnormal	Fig. 8

1 - 10 of 15

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
hi1321Tg	Tg(F5)	Transgenic Insertion	foxi1
hi1359aTg	Tg(nLacz-GTvirus)	Transgenic Insertion	foxi1
hi3239Tg	Tg(nLacz-GTvirus)	Transgenic Insertion	foxi1
hi3747Tg	Tg(nLacz-GTvirus)	Transgenic Insertion	foxi1

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
fgf3	MO1-fgf3	MRPHLNO
foxi1	MO4-foxi1	MRPHLNO

Fish

Fish
foxi1^hi1321Tg
foxi1^hi1359aTg
foxi1^hi3239Tg
foxi1^hi3747Tg
WT
WT + MO1-fgf3

Antibodies

Orthology

Gene	Orthology
foxi1

Engineered Foreign Genes

Mapping

Nissen et al., 2003 ZDB-PUB-030425-24 PMID:12702667

Zebrafish foxi one modulates cellular responses to Fgf signaling required for the integrity of ear and jaw patterning

Nissen et al., 2003

ZDB-PUB-030425-24
PMID:12702667