PUBLICATION

Roles for zebrafish focal adhesion kinase in notochord and somite morphogenesis

Authors
Henry, C.A., Crawford, B.D., Yan, Y.-L., Postlethwait, J., Cooper, M.S., and Hille, M.B.
ID
ZDB-PUB-011113-1
Date
2001
Source
Developmental Biology   240(2): 474-487 (Journal)
Registered Authors
Cooper, Mark S., Crawford, Bryan D., Henry, Clarissa A., Hille, Merrill, Postlethwait, John H., Yan, Yi-Lin
Keywords
focal adhesion kinase; phosphorylation; zebrafish; notochord; somitogenesis; Notch; knypek; trilobite; fused-somite
MeSH Terms
  • Gene Expression Regulation, Developmental
  • DNA, Complementary/genetics
  • Protein-Tyrosine Kinases/chemistry
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/physiology*
  • Sequence Homology, Amino Acid
  • Somites/cytology
  • Somites/enzymology
  • Gene Expression Regulation, Enzymologic
  • Base Sequence
  • Notochord/embryology*
  • Notochord/enzymology
  • Molecular Sequence Data
  • Phosphorylation
  • Mutation
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Focal Adhesion Protein-Tyrosine Kinases
  • Chromosome Mapping
  • Signal Transduction
  • Receptors, Notch
  • Amino Acid Sequence
  • Membrane Proteins/physiology
  • Cloning, Molecular
  • Morphogenesis
  • Body Patterning
(all 30)
PubMed
11784077 Full text @ Dev. Biol.
Abstract
We have cloned zebrafish focal adhesion kinase (Fak) and analyzed its subcellular localization. Fak protein is localized at the cortex of notochord cells and at the notochord-somite boundary. During somitogenesis, Fak protein becomes concentrated at the basal region of epithelial cells at intersomitic boundaries. Phosphorylated Fak protein is seen at both the notochord-somite boundary and intersomitic boundaries, consistent with a role for Fak in boundary formation and maintenance. The localization of Fak protein to the basal region of epithelial cells in knypek;trilobite double mutant embryos shows that polarization of Fak distribution in the somite border cells is independent of internal mesenchymal cells. In addition, we show that neither Notch signaling through Suppressor of Hairless (SuH) nor deltaD is necessary for the wild-type segmental pattern of fak mRNA expression in the anterior paraxial mesoderm. However, nonsegmental expression of fak mRNA occurs with ectopic activation of Notch signaling through SuH and also in fused somite and beamter mutant embryos, indicating that there are multiple regulators of fak mRNA expression. Our results suggest that Fak plays a central role in notochord and somite morphogenesis.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
ptk2abWTstandard conditions10-13 somitesISH
ptk2abWTstandard conditions14-19 somitesISH
ptk2abWTstandard conditions1-4 somitesISH
ptk2abWTstandard conditions5-9 somitesISH
ptk2abWTstandard conditionsPrim-5ISH
ptk2abWTstandard conditions10-13 somitesISH
ptk2abWTstandard conditions1-4 somitesISH
ptk2abWTstandard conditions5-9 somitesISH
ptk2abWTstandard conditions10-13 somitesISH
ptk2abWTstandard conditions14-19 somitesISH
1 - 10 of 11
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    b195
      		Insertion
      n1
        		Small Deletion
        te314a
          		Point Mutation
          to202
            		Point Mutation
            tr233
              		Point Mutation
              tx201
                		Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                Fish
                dlcto202/to202
                dldtr233/tr233
                notch1atx201/tx201
                noton1/n1 (AB)
                tbx6te314a/te314a
                tbx16b104/b104 (AB)
                tbxtab195/b195
                WT
                1 - 8 of 8
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                Antibodies
                Name Type Antigen Genes Isotypes Host Organism
                Ab1-ptk2polyclonalIgGRabbit
                Ab1-ptk2.1polyclonalIgGRabbit
                1 - 2 of 2
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                Orthology
                Gene Orthology
                ptk2ab
                1 - 1 of 1
                Show
                Engineered Foreign Genes
                No data available
                Mapping
                Entity Type Entity Symbol Location
                ESTfb52a02Chr: 16 Details
                ESTzf-a41Chr: 16 Details
                GENEptk2abChr: 16 Details
                1 - 3 of 3
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                Citation
                Henry, C.A., Crawford, B.D., Yan, Y.-L., Postlethwait, J., Cooper, M.S., and Hille, M.B. (2001) Roles for zebrafish focal adhesion kinase in notochord and somite morphogenesis. Developmental Biology. 240(2):474-487.