PUBLICATION

Roles for zebrafish focal adhesion kinase in notochord and somite morphogenesis

Authors
Henry, C.A., Crawford, B.D., Yan, Y.-L., Postlethwait, J., Cooper, M.S., and Hille, M.B.
ID
ZDB-PUB-011113-1
Date
2001
Source
Developmental Biology   240(2): 474-487 (Journal)
Registered Authors
Cooper, Mark S., Crawford, Bryan D., Henry, Clarissa A., Hille, Merrill, Postlethwait, John H., Yan, Yi-Lin
Keywords
focal adhesion kinase; phosphorylation; zebrafish; notochord; somitogenesis; Notch; knypek; trilobite; fused-somite
MeSH Terms
  • Phosphorylation
  • Somites/cytology
  • Somites/enzymology
  • Animals
  • Chromosome Mapping
  • Base Sequence
  • Cloning, Molecular
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Membrane Proteins/physiology
  • Body Patterning
  • Receptors, Notch
  • DNA, Complementary/genetics
  • Signal Transduction
  • Amino Acid Sequence
  • Gene Expression Regulation, Developmental
  • Notochord/embryology*
  • Notochord/enzymology
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Morphogenesis
  • Focal Adhesion Protein-Tyrosine Kinases
  • Mutation
  • Gene Expression Regulation, Enzymologic
  • Protein-Tyrosine Kinases/chemistry
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
(all 30)
PubMed
11784077 Full text @ Dev. Biol.
Abstract
We have cloned zebrafish focal adhesion kinase (Fak) and analyzed its subcellular localization. Fak protein is localized at the cortex of notochord cells and at the notochord-somite boundary. During somitogenesis, Fak protein becomes concentrated at the basal region of epithelial cells at intersomitic boundaries. Phosphorylated Fak protein is seen at both the notochord-somite boundary and intersomitic boundaries, consistent with a role for Fak in boundary formation and maintenance. The localization of Fak protein to the basal region of epithelial cells in knypek;trilobite double mutant embryos shows that polarization of Fak distribution in the somite border cells is independent of internal mesenchymal cells. In addition, we show that neither Notch signaling through Suppressor of Hairless (SuH) nor deltaD is necessary for the wild-type segmental pattern of fak mRNA expression in the anterior paraxial mesoderm. However, nonsegmental expression of fak mRNA occurs with ectopic activation of Notch signaling through SuH and also in fused somite and beamter mutant embryos, indicating that there are multiple regulators of fak mRNA expression. Our results suggest that Fak plays a central role in notochord and somite morphogenesis.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-ptk2polyclonalIgGRabbit
Ab1-ptk2.1polyclonalIgGRabbit
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Orthology
Gene Orthology
ptk2ab
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Engineered Foreign Genes
No data available
Mapping
Entity Type Entity Symbol Location
ESTfb52a02Chr: 16 Details
ESTzf-a41Chr: 16 Details
GENEptk2abChr: 16 Details
1 - 3 of 3
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