PUBLICATION

Roles for zebrafish focal adhesion kinase in notochord and somite morphogenesis

Authors
Henry, C.A., Crawford, B.D., Yan, Y.-L., Postlethwait, J., Cooper, M.S., and Hille, M.B.
ID
ZDB-PUB-011113-1
Date
2001
Source
Developmental Biology   240(2): 474-487 (Journal)
Registered Authors
Cooper, Mark S., Crawford, Bryan D., Henry, Clarissa A., Hille, Merrill, Postlethwait, John H., Yan, Yi-Lin
Keywords
focal adhesion kinase; phosphorylation; zebrafish; notochord; somitogenesis; Notch; knypek; trilobite; fused-somite
MeSH Terms
  • DNA, Complementary/genetics
  • Notochord/embryology*
  • Notochord/enzymology
  • Focal Adhesion Protein-Tyrosine Kinases
  • Somites/cytology
  • Somites/enzymology
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Body Patterning
  • Mutation
  • Gene Expression Regulation, Enzymologic
  • Phosphorylation
  • Chromosome Mapping
  • Receptors, Notch
  • Protein-Tyrosine Kinases/chemistry
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/physiology*
  • Morphogenesis
  • Membrane Proteins/physiology
  • Gene Expression Regulation, Developmental
  • Sequence Homology, Amino Acid
  • Amino Acid Sequence
  • Base Sequence
  • Animals
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Cloning, Molecular
(all 30)
PubMed
11784077 Full text @ Dev. Biol.
Abstract
We have cloned zebrafish focal adhesion kinase (Fak) and analyzed its subcellular localization. Fak protein is localized at the cortex of notochord cells and at the notochord-somite boundary. During somitogenesis, Fak protein becomes concentrated at the basal region of epithelial cells at intersomitic boundaries. Phosphorylated Fak protein is seen at both the notochord-somite boundary and intersomitic boundaries, consistent with a role for Fak in boundary formation and maintenance. The localization of Fak protein to the basal region of epithelial cells in knypek;trilobite double mutant embryos shows that polarization of Fak distribution in the somite border cells is independent of internal mesenchymal cells. In addition, we show that neither Notch signaling through Suppressor of Hairless (SuH) nor deltaD is necessary for the wild-type segmental pattern of fak mRNA expression in the anterior paraxial mesoderm. However, nonsegmental expression of fak mRNA occurs with ectopic activation of Notch signaling through SuH and also in fused somite and beamter mutant embryos, indicating that there are multiple regulators of fak mRNA expression. Our results suggest that Fak plays a central role in notochord and somite morphogenesis.
Genes / Markers
Figures
Figure Gallery (7 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    b195
      		Insertion
      n1
        		Small Deletion
        te314a
          		Point Mutation
          to202
            		Point Mutation
            tr233
              		Point Mutation
              tx201
                		Insertion
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                Fish
                dlcto202/to202
                dldtr233/tr233
                notch1atx201/tx201
                noton1/n1 (AB)
                tbx6te314a/te314a
                tbx16b104/b104 (AB)
                tbxtab195/b195
                WT
                1 - 8 of 8
                Show
                Antibodies
                Name Type Antigen Genes Isotypes Host Organism
                Ab1-ptk2polyclonalIgGRabbit
                Ab1-ptk2.1polyclonalIgGRabbit
                1 - 2 of 2
                Show
                Orthology
                Gene Orthology
                ptk2ab
                1 - 1 of 1
                Show
                Engineered Foreign Genes
                No data available
                Mapping
                Entity Type Entity Symbol Location
                ESTfb52a02Chr: 16 Details
                ESTzf-a41Chr: 16 Details
                GENEptk2abChr: 16 Details
                1 - 3 of 3
                Show
                Your Input Welcome
                We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                Citation
                Henry, C.A., Crawford, B.D., Yan, Y.-L., Postlethwait, J., Cooper, M.S., and Hille, M.B. (2001) Roles for zebrafish focal adhesion kinase in notochord and somite morphogenesis. Developmental Biology. 240(2):474-487.