PUBLICATION

Hedgehog signalling is required for maintenance of myf5 and myoD expression and timely terminal differentiation in zebrafish adaxial myogenesis

Authors

Coutelle, O., Blagden, C.S., Hampson, R., Halai, C., Rigby, P.W.J., and Hughes, S.M.

ID

ZDB-PUB-010705-7

Date

2001

Source

Developmental Biology 236(1): 136-150 (Journal)

Registered Authors

Blagden, Chris, Hampson, Richard, Hughes, Simon M.

Keywords

skeletal muscle; zebrafish; myf5; myoD; hedgehog; differentiation; somite; myoblast; myogenesis

MeSH Terms

Myogenic Regulatory Factor 5
Zebrafish
In Situ Hybridization
In Situ Nick-End Labeling
Molecular Sequence Data
Sequence Homology, Amino Acid
Muscles/embryology
Cell Survival
Mutation
Cell Lineage
Immunohistochemistry
MyoD Protein/biosynthesis*
Animals
Time Factors
Up-Regulation
Proteins/metabolism*
Hedgehog Proteins
Trans-Activators*
Signal Transduction*
Cell Differentiation
Cloning, Molecular
DNA-Binding Proteins*
Notochord/metabolism
Amino Acid Sequence
Muscle Proteins/biosynthesis*
Phenotype

(all 26)

PubMed

11456450 Full text @ Dev. Biol.

Abstract

Hedgehog proteins have been implicated in the control of myogenesis in the medial vertebrate somite. In the mouse, normal epaxial expression of the myogenic transcription factor gene myf5 is dependent on Sonic hedgehog. Here we examine in zebrafish the interaction between Hedgehog signals, the expression of myoD family genes, including the newly cloned zebrafish myf5, and slow myogenesis. We show that Sonic hedgehog is necessary for normal expression of both myf5 and myoD in adaxial slow muscle precursors, but not in lateral paraxial mesoderm. Expression of both genes is initiated normally in rostral presomitic mesoderm in sonic you mutants, which lack all Sonic hedgehog. Similar initiation continues during tailbud outgrowth when the cells forming caudal somites are generated. However, adaxial cells in sonic you embryos are delayed in terminal differentiation and caudal adaxial cells fail to maintain myogenic regulatory factor expression. Despite these defects, other signals are able to maintain, or reinitiate, some slow muscle development in sonic you mutants. In the cyclops mutant, the absence of floorplate-derived Tiggywinkle hedgehog and Sonic hedgehog has no discernible effect on slow adaxial myogenesis. Similarly, the absence of notochord-derived Sonic hedgehog and Echidna hedgehog in mutants lacking notochord delays, but does not prevent, adaxial slow muscle development. In contrast, removal of both Sonic hedgehog and a floorplate signal, probably Tiggywinkle hedgehog, from the embryonic midline in cyclops;sonic you double mutants essentially abolishes slow myogenesis. We conclude that several midline signals, likely to be various Hedgehogs, collaborate to maintain adaxial slow myogenesis in the zebrafish embryo. Moreover, the data demonstrate that, in the absence of this required Hedgehog signalling, expression of myf5 and myoD is insufficient to commit cells to adaxial myogenesis.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
myf5	noto^n1/n1	standard conditions	Bud	adaxial cell	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	5-9 somites	adaxial cell	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	10-13 somites	adaxial cell	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	Bud	paraxial mesoderm	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	10-13 somites	segmental plate	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	5-9 somites	segmental plate	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	5-9 somites	somite	ISH	Fig. 4
myf5	noto^n1/n1	standard conditions	10-13 somites	somite	ISH	Fig. 4
myf5	tbxta^b160/b160	standard conditions	14-19 somites	segmental plate	ISH	Fig. 5
myf5	tbxta^b160/b160	standard conditions	5-9 somites	segmental plate	ISH	Fig. 5

1 - 10 of 79

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-5	slow muscle cell normal amount, normal	Fig. 6
noto^n1/n1	standard conditions	10-13 somites	adaxial cell mislocalised, abnormal	Fig. 4
noto^n1/n1	standard conditions	14-19 somites	adaxial cell immature, abnormal	Fig. 4
noto^n1/n1	standard conditions	14-19 somites	striated muscle cell differentiation delayed, abnormal	Fig. 4
shha^{tbx392/tbx392}	standard conditions	Bud	adaxial cell physical object quality, abnormal	Fig. 1
shha^{tbx392/tbx392}	standard conditions	Bud	skeletal muscle fiber development process quality, abnormal	Fig. 1
shha^{tbx392/tbx392}	standard conditions	14-19 somites	adaxial cell immature, abnormal	Fig. 4
shha^{tbx392/tbx392}	standard conditions	14-19 somites	adaxial cell physical object quality, abnormal	Fig. 1
shha^{tbx392/tbx392}	standard conditions	14-19 somites	skeletal muscle fiber development process quality, abnormal	Fig. 1
shha^{tbx392/tbx392}	standard conditions	14-19 somites	striated muscle cell differentiation delayed, abnormal	Fig. 4

1 - 10 of 23

Show

Mutations / Transgenics

Allele	Type	Affected Genomic Region
b16	Deficiency	hhex ndr2
b160	Indel	tbxta
n1	Small Deletion	noto
tbx392	Point Mutation	shha

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
Df(Chr12:hhex,ndr2)b16/b16
noto^n1/n1
shha^{tbx392/tbx392}
shha^{tbx392/tbx392}; Df(Chr12:hhex,ndr2)b16/b16
tbxta^b160/b160
WT

Antibodies

Name	Type	Isotypes	Host Organism
Ab1-smyhc	monoclonal	IgG2b	Mouse
Ab-A4.1025	monoclonal	IgG2a	Mouse
Ab-EB165	monoclonal	IgG1	Mouse

Orthology

Gene	Orthology
myf5

Engineered Foreign Genes

Mapping

Coutelle et al., 2001 ZDB-PUB-010705-7 PMID:11456450

Hedgehog signalling is required for maintenance of myf5 and myoD expression and timely terminal differentiation in zebrafish adaxial myogenesis

Coutelle et al., 2001

ZDB-PUB-010705-7
PMID:11456450