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Fig. 5 In vivo imaging of zebrafish brains using a confocal microscope (A) Photograph of a 60-mm plastic dish with a 14-mm glass coverslip-filled well. (B) Photographs the larvae embedded in the glass coverslip-filled well of a 60-mm plastic dish. (C) Schematic showing the operation that add ES or Hank’s solution (marked as blue) for the embedded larvae before imaging. (D) Photograph of a FV3000 confocal microscope (Olympus) for in vivo imaging of zebrafish. (E) Screenshot of the software interface (FV31S-SW) for operating the FV3000. The red box marks the settings for scan speed, resolution, aperture, laser intensity, detector voltage, z-series, and z-step positions. (F) Representative images of pericytes and blood vessels in the brain of Ki(pdgfrb-P2A-Gal4-VP16);Tg(4×nrUAS:GFP);Tg(fli1a:DsRed) larvae. These images are processed with the software ImageJ. Pericyte in green, endothelial cell in red. Scale bar 50 μm.