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Fig. 6 bmp5 is expressed in and around the developing enteric progenitors during ENS development. Whole-mount immuno-coupled hybridization chain reaction analysis of novel ENS-related BMP ligand transcript, bmp5, in combination with neuronal markers Phox2b and Elavl3/4 at 24 hpf (A–D′), 36 hpf (E–H′), 48 hpf (I–L′) and 96 hpf (M–P). Scale bar in A, E, I and M denotes 70 microns. OV, otic vesicle. green arrows denote colocalization. ≥ 3 experiments with three biological replicates.
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