FIGURE 3

FIGURE 3

Depletion of insulin production results in defective rheotaxis behavior, but not locomotor behavior, in ins:epNTR-mCherry zebrafish. (A) Quantification of the distribution of the Ins:epNTR-mCherry larvae performing rheotactic angles following treatment with DMSO and MTZ, and siblings treated with MTZ at 9 dpf, ***p = 0.0004, Chi-squared test (n = 15 larvae per group). (B) Quantification of the percentage of the transgenic larvae showing rheotactic behavior following treatment with DMSO and MTZ, and siblings with MTZ at 9 dpf, **p = 0.0064, Chi-squared test (n = 15 larvae per group). (C) Tracking visualization of the transgenic larvae performing free locomotion in light state at 9 dpf. (D,E) Quantification of the distance moved (mm) (D) and velocity (mm/s) (E) from the transgenic larvae performing free locomotion under light conditions following treatment with DMSO and MTZ, and sibling with MTZ at 9 dpf (n = 32 larvae per group).