Live staining of 5 dpf zebrafish embryos with DAF-FM DA for short intervals. Column (A): live imaging of the head region at the time indicated on the left; inset: magnification of the opercular bone. The strong blurred signal visible ventrally is the bulbus arteriosus. Scale bars = 200 µm. Columns (B–D): 3 µm GMA sections of the corresponding embryos. Column (B): bulbus arteriosus. Scale bars = 10 µm. (C): notochord in the head region. Scale bars = 20 µm except for 60 min: 10 µm. (D): opercular bone. Scale bars = 10 µm except for 40 min: 20 µm. Column (A): anterior to the left; (B,C): dorsal to the top; (D): lateral to the left.
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