Figure 4

Assessment of purine deoxynucleoside supplementation in 5 dpf rrm2b larvae—.(A) Relative mtDNA copy number in whole 5 dpf rrm2b larvae, treated with either vehicle (DMSO) (rrm2b^+/+ n = 7 (circles with thin border), rrm2b^−/− n = 9, (squares with thin border)) or purine deoxynucleosides (50 μM dAdo & dGuo + 5 μM ENHA in 0.5%DMSO) (rrm2b^+/+ n = 4 (circles with thick border), rrm2b^−/− n = 10 (squares with thick border)) between 0 dpf and 5 dpf. Results are shown as mean ± SEM. (B) Average movement profile of 5 dpf vehicle treated rrm2b^+/+ (0.5% DMSO vehicle)(n = 22 (circles with thin border)), vehicle treated rrm2b^−/− (0.5% DMSO vehicle)(n = 17 (squares with thin border)) and deoxynucleoside treated rrm2b^−/− (50 μM dAdo & dGuo + 5 μM ENHA in 0.5%DMSO)(n = 21 (squares with thick border)) larvae during a light dark transition test (LDT). (C) Total distance moved in light periods of vehicle treated 5 dpf rrm2b^+/+ larvae (n = 22 (circles with thin border)), vehicle treated rrm2b^−/− (0.5% DMSO vehicle)(n = 17 (squares with thin border)) and deoxynucleoside treated rrm2b^−/− (50 μM dAdo & dGuo + 5 μM ENHA in 0.5%DMSO)(n = 21 (squares with thick border)) larvae during an LDT test. (I) Total distance moved in dark periods of vehicle treated 5 dpf rrm2b^+/+ larvae (n = 22 (circles with thin border), vehicle treated rrm2b^−/− (0.5% DMSO vehicle)(n = 17 (squares with thin border) and deoxynucleoside treated rrm2b^−/− (50 μM dAdo & dGuo + 5 μM ENHA in 0.5%DMSO)(n = 21 (squares with thick border)) larvae during an LDT test. Results are shown as mean ± SEM (statistical significance was determined with a one-way ANOVA, ^**P < 0.01).