Phagocytosis of hypovirulent C. albicans mutants. (A) Example representative images from nmd5∆/∆, rim101∆/∆ and mad2∆/∆-infected fish, along with SN250-infected controls, at 4–6 hours postinfection. Images were scored by eye for the number of macrophages (Mpeg1-GFP+ cells) shown in green and the number of neutrophils (LysC-dsRed+ cells) in magenta recruited to the infection, as well as if the Candida was intracellular or extracellular. Scale bar is 100 µm. (B–D) Quantification of phagocytosis-related phenotypes. There are separate SN250 columns for each set of experiments, as the mutant was compared to wild type in the same experiments. (B) Plots of the percent intracellular Candida normalized to the average percent intracellular Candida for SN250. (C) Plots showing the number of extracellular Candida normalized to the average amount for SN250. (D) Plots showing the number of intracellular Candida, divided by the number of cells recruited, normalized to the average for SN250. (B–D) Shading indicates Groups I–IV based on similar interaction phenotypes (Fig. 4). Means and 95% confidence intervals are plotted. Statistics were performed from pooled data from at least three independent experiments for each mutant, for approximately 25 fish per strain imaged. Hedges bias-corrected effect sizes and significance were determined for each mutant. * indicates P < 0.05, # indicates a moderate effect, while a bold # indicates a strong effect.
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