FIGURE

Fig.5

ID

ZDB-FIG-250122-64

Publication

Ranard et al., 2024 - Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment

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Fig.5

Maternal dietary DHA restriction and biosynthesis disruption depletes offspring DHA and increases ARA. Adult wild-type and elovl2^+/− parents were fed either SUFF or DEF for 25–29 weeks prior to spawning. A: DHA and (B) ARA concentrations in 0 hpf embryos and 4 dpf larvae measured by GC/MS. Offspring from elovl2-DEF mothers had similar fatty acid status, regardless of +/+ or −/− genotype. Dots represent individually analyzed offspring (n = 6–12 fish/group). Groups labeled with different letters denote significant differences by 1-way ANOVA with Tukey’s post-hoc test (P < 0.05). C: Representative DHA-PL and ARA-PL species in 4 dpf larvae measured by LC/MS/MS (n = 3 pooled replicates/group; n = 3 larvae per pool). Values are log₂(fold change) relative to the WT-SUFF group mean. DHA-PLs decreased and ARA-PLs increased in offspring from DEF-fed mothers compared to the WT-SUFF group. ARA, arachidonic acid; DHA, docosahexaenoic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PL, phospholipid; PS, phosphatidylserine.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB elovl2^co100/co100 (AB)
Condition:	low polyunsaturated fat
Observed In:	whole organism arachidonic acid whole organism docosahexaenoic acid
Stage Range:	1-cell to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.