FIGURE

Fig. 1

ID

ZDB-FIG-240305-1

Publication

Schorpp et al., 2023 - Foxn1 is not essential for T-cell development in teleosts

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Fig. 1

Characterization of a foxn1 null allele in zebrafish. (A) Schematic structure of the zebrafish foxn1 gene; exons are numbered, and the exons encoding the DNA binding domain are indicated as red rectangles. A double-headed arrow below the schematic indicates the region deleted in the foxn1fr107 mutant allele. (B) Partial sequence of the cDNA spanning the deletion from within exon 2 to within exon 6; note the insertion of seven nucleotides as a result of the CRISPR/Cas9-mediated rearrangement that fuses parts of exons 2 and 6. (C) RT-PCR analysis of the indicated epithelial genes in the thymi of wildtype (wt) and foxn1-deficient (mut) zebrafish (M; marker lane; amplicon sizes: pax9, 149 bp; foxn1, 110 bp; foxn4, 174 bp. Note the absence of an intact foxn1 transcript in the mutant thymus.

Expression Data

Genes:	foxn1 foxn4 pax9
Fish:	TU foxn1^fr107/fr107 (TU)
Anatomical Term:	thymus
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxn1^fr107/fr107 (TU)
Observed In:	thymus
Stage:	Days 30-44

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Immunol.