FIGURE

Fig 4

ID

ZDB-FIG-210802-16

Publication

Wen et al., 2021 - The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics

Other Figures

(all 8)

All Figure Page

Back to All Figure Page

Fig 4

<italic toggle='yes'>Klf6a</italic> regulates EC nucleus migration in CV pruning.

(A) Time-lapse live imaging of Tg(fli1a:nEGFP;kdrl:mCherry) zebrafish embryos shows EC nucleus migration in CV pruning in control and klf6a morphant. The arrow shows the direction of the blood flow. Colored circles indicate EC nuclei. Scale bar: 10 μm. (B) The direction of EC migration within the regressing vessel. The direction is classified into three types: with the flow, static, and against the flow. A total of 10 vascular loops with 14 EC nuclei and 14 vascular loops with 21 EC nuclei were calculated in the control group and klf6a morphant group, respectively. P = 0.0058. Unpaired two-tailed chi-square test. (C) and (D) Efficiency of siKLF6 in human umbilical vein endothelial cells (HUVECs) at the mRNA (P = 0.00087) and protein (P = 0.0003) levels. (E) Wound healing of siCTR-transfected and siKLF6-transfected HUVECs: 6 h, P = 0.0013; 12 h, P = 0.0020. (C) to (E) Student’s unpaired two-tailed t test. *P < 0.05, **P < 0.01, ***P < 0.001.

Expression Data

Genes:	EGFP mCherry
Fish:	ci5Tg; y7Tg ci5Tg; y7Tg + MO1-klf6a
Knockdown Reagent:	MO1-klf6a
Anatomical Terms:	caudal vein plexus caudal vein plexus nucleus
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ci5Tg; y7Tg + MO1-klf6a
Knockdown Reagent:	MO1-klf6a
Observed In:	caudal vein plexus blood vessel endothelial cell migration
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.