Fig. 4
- ID
- ZDB-FIG-180910-40
- Publication
- Bekri et al., 2018 - Glycine Promotes the Survival of a Subpopulation of Neural Stem Cells
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Glycine signaling defects induces a drastic loss of nestin+ NSCs subpopulation. (A) Using nestin as a marker of NSCs subpopulation, nestin transcripts were analyzed by whole-mount in situ hybridization. A drastic loss of nestin+ subpopulation was observed between Ctrl-MO (i), and Glr-MO (ii) conditions at 15 and 24 hpf. (B) Double tg(GFAP:GFP; HuC:RFP) line was used to analysis nestin (white) and HuC+ (Bleu) expressions. Major reduction of nestin+ signals in Glr-MO conditions (ii) compared with Ctrl-MO (i) at 24 and 48 hpf. In addition to reduction of HuC+ signals in Glr-MO condition (ii). (C) Quantification of nestin-GFP embryos upon disruption of glycine signaling reveal a drastic reduction of GFP signal compared with control. However, co-injecting of p53-MO and Glr-MO rescue partially nestin+ subpopulation. For both ISH and IHC, n > 20 embryos per sample. IHC, immunohistochemistry; ISH, in situ hybridization. |
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Anatomical Terms: | (all 6) |
Stage Range: | 10-13 somites to Long-pec |
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Knockdown Reagent: | |
Observed In: | |
Stage Range: | 10-13 somites to Long-pec |