FIGURE

Fig. S1

ID

ZDB-FIG-180828-23

Publication

Gauvrit et al., 2018 - HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development

Other Figures

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Fig. S1

zebrafish hhex mutants exhibit a delay during PHBC formation but form arterial intersegmental vessels

(a-b) Maximum intensity projections of confocal images of 24 hpf Tg(fli:nEGFP); hhex^+/+ and hhex^-/- embryos after GFP immunostaining. hhex mutants exhibit delayed primordial hindbrain channels (PHBCs) formation (arrowheads). (c-d) Maximum intensity projections of confocal images of 48 hpf TgBAC(etv2:EGFP); hhex^+/+ and hhex^-/- embryos. hhex mutants form arterial ISVs. (e-h) Trunk vasculature of 5 dpf Tg(kdrl:EGFP); Tg(-5.2lyve1b:DsRed); hhex^+/+ and hhex^-/-. hhex mutants form arterial ISVs while vessels from the PCV (vISVs and TD) are mostly absent (arrowheads point to the ventrally positioned TD; asterisks indicate lack of this structure). i) Quantification of vISVs across 10 somites in 5 dpf hhex^+/? (n=8) and hhex^-/- (n=8). Values represent means ± s.e.m. ****P ≤0.0001 by t-test. Scale bars: 100 μm (a-b), 200 μm (c-d), 50 μm (e-h).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hhex^s721/s721; ci1Tg hhex^s721/s721; nz101Tg; s843Tg hhex^s721/s721; y7Tg hhex^s722/s722; ci1Tg hhex^s722/s722; nz101Tg; s843Tg hhex^s722/s722; y7Tg (all 6)
Observed In:	intersegmental artery intersegmental vein lymph vessel endothelium posterior cardinal vein sprouting angiogenesis primordial hindbrain channel somite intersegmental vein thoracic duct intersegmental vessel (all 7)
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.