FIGURE

Fig. 3

ID

ZDB-FIG-180828-20

Publication

Gauvrit et al., 2018 - HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development

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Fig. 3

Zebrafish hhex mutants lack lymphatic precursors. a–d Whole-mount views of 36 hpf hhex^+/+ and hhex^−/− embryos immunostained for Prox1 (arrowheads point to Prox1⁺ endothelial cells in the PCV). e Quantification of the number of Prox1⁺ endothelial cells across three somites (from two field of view per embryo) in hhex^+/? (n = 8) and hhex^−/− (n = 6). f–i Whole-mount views of 36 hpf Tg(prox1a:TagRFP); hhex^+/+ and hhex^−/− embryos. hhex mutants exhibit a strong decrease in the number of RFP⁺ cells in the PCV compared to hhex^+/+ (arrowheads point to RFP⁺ endothelial cells in the PCV). j Quantification of the number of RFP⁺ endothelial cells across three somites (from two field of view per embryo) in hhex^+/? (n = 10) and hhex^−/− (n = 4). Values represent means ± s.e.m. ***P ≤ 0.001, and **P ≤ 0.01 by t-test. Scale bars: 50 μm

Expression Data

Gene:	TagRFP
Antibody:	Ab7-prox1
Fish:	hhex^s722/s722 hhex^s722/s722; nim5Tg; s843Tg
Anatomical Term:	endothelial cell
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hhex^s722/s722 hhex^s722/s722; nim5Tg hhex^s722/s722; nim5Tg; s843Tg
Observed In:	endothelial cell posterior cardinal vein endothelial cell somite endothelial cell
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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