FIGURE

Fig. 4

ID
ZDB-FIG-180418-19
Publication
Sarmah et al., 2017 - Embryonic Ethanol Exposure Affects Early- and Late-Added Cardiac Precursors and Produces Long-Lasting Heart Chamber Defects in Zebrafish
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Fig. 4

Embryonic ethanol exposure reduced second heart field precursors. (A–I) Anti-Islet1 antibody stained Tg(myl7:GFP) embryos showed Islet1 positive second heart field precursors in the rotating heart cone in control embryos (A–C) at 22 hpf; ethanol exposed embryos showed reduced number of Islet1 positive cells in the heart cones (D–I) at 22 hpf; (J–R) Anti-Islet1 antibody stained Tg(myl7:GFP) embryos showed second heart field derived cardiomyoctes in the heart (Myl7 and Islet1 double positive; red and green) and second heart field precursors (Islet1 positive, red) near the out flow pole in the control embryos (J–L) and ethanol-exposed embryos (M–R) at 28 hpf; (S) Graph shows the quantification of the Islet1/Myl7 double positive cardiomyocytes in the heart tube and Islet1 positive cells near the outflow pole at 28 hpf.

Expression Data
Gene:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage Range: 26+ somites to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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