FIGURE

Fig. 6

ID

ZDB-FIG-160609-19

Publication

Steed et al., 2016 - klf2a couples mechanotransduction and zebrafish valve morphogenesis through fibronectin synthesis

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Fig. 6

Characterization of klf2a^-/- mutants.

(a) anti-fibronectin staining (green) is reduced in the AVC of trpp2^-/- mutants (12/12 trpp2^+/+, 9/19 trpp2^-/-). (b,c) A range of valvular defects were observed in klf2a^-/- mutant embryos at 96 hpf (n=25 klf2a^+/+, n=48 klf2a^-/-), similar to those observed in klf2aMO-injected embryos (e; n=20 control, n=17 klf2aMO). (d) Photoconversion of AVC EdCs at 48 hpf shows the organization of cells within the cardiac jelly to be affected by loss of klf2a (d,e; n=21 klf2^+/+, n=32 klf2a^-/-) at 72 hpf. Scale bars, 10 µm.

Expression Data

Genes:	Kaede mCherry
Antibody:	Ab2-fn
Fish:	ubs10Tg rk8Tg; ubs3Tg klf2a^ig4/ig4; rk8Tg; ubs3Tg pkd2^tc321/tc321; ubs10Tg
Anatomical Terms:	atrioventricular canal atrium cardiac jelly cardiac ventricle endocardium endothelial cell heart (all 6)
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	klf2a^ig4/ig4 (AB) AB + MO3-klf2a AB + MO4-klf2a klf2a^ig4/ig4; rk8Tg; ubs3Tg pkd2^tc321/tc321; ubs10Tg
Knockdown Reagents:	MO3-klf2a MO4-klf2a
Observed In:	atrioventricular canal atrioventricular valve atrioventricular valve development atrioventricular valve morphogenesis cardiac jelly cell
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.