Fig. 4 S3
- ID
- ZDB-FIG-160215-37
- Publication
- Yokota et al., 2015 - Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo
- Other Figures
- (all 27)
- All Figure Page
- Back to All Figure Page
PlexinD1 is necessary to confine Ca2+-oscillating sprouts in the vicinity of somite boundaries. (A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos during tip cell budding injected with plxnD1 MO (18 ss). Arrowheads indicate Ca2+-oscillating ECs budding from the DA. Yellow dashed lines indicate positions of somite boundaries. Note that plxnD1 morphants display ectopic EC sprouts that exhibit Ca2+ oscillations. (B) Fluorescence changes in GCaMP7a (ΔF/F0) of individual ECs indicated by arrowheads in A are shown as a graph. Scale bar, 10 mm in A. |