FIGURE

Fig. 9

ID

ZDB-FIG-131009-29

Publication

Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling

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Fig. 9

Vagal neurons are sensitive to alterations in retinoic acid levels.Tg(isl1:eGFP) transgenic zebrafish are used to visualize alterations to branchiomotor neurons in response to 1% DMSO (A, D), 5 nM all-trans retinoic acid (B, E), or 5 μM DEAB (C, F) treatment. Images are dorsal views of 48 hpf embryo hindbrain using 20× or (A-C) or 40× (D-F) objective. Length (G) and area (H) were quantified using ImageJ and displayed graphically (error bar denotes standard deviation). The differences induced by either retinoic acid or DEAB treatment are statistically significant (ANOVA and Tukey’s HSD post-hoc test, *p-value < 0.01).

Expression Data

Gene:	GFP
Fish:	rw0Tg
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Anatomical Terms:	branchiomotor neuron facial nerve motor nucleus motor nucleus of vagal nerve trigeminal motor nucleus
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Observed In:	motor nucleus of vagal nerve anatomical region
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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