Fig. S17
- ID
- ZDB-FIG-120824-6
- Publication
- Chen et al., 2012 - Haemodynamics-driven developmental pruning of brain vasculature in zebrafish
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Method for tracing fate of each vessel segment in the midbrain. (A and B) Projected confocal images (left) and 3-D reconstructions (right) of half midbrain vasculature in a Tg(kdrl:eGFP) zebrafish larvae at 2.5 dpf (A) or 3 dpf (B). Colored balls mark different branch points. The dashed circle marks the site at which a branch point will appear at the next imaging time point. The corresponding movies of the 3-D rotation centerlines are shown in Video S7. Green, newly formed segments; red, pruning segments; blue, CVP. Scale, 50 μm. |