FIGURE

Fig. 5

ID

ZDB-FIG-120405-19

Publication

Jao et al., 2012 - A zebrafish model of lethal congenital contracture syndrome 1 reveals Gle1 function in spinal neural precursor survival and motor axon arborization

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Fig. 5

Secondary motoneurons are severely reduced in gle1 morphants. (A-F) Confocal images of control (A-C) and gle1 morphant (D-F) zebrafish embryos with motoneurons labeled by mnx1:GFP transgene and zn-8 (Alcama) antibody to reveal somata and fasciculated axons of secondary motoneurons at 56 hpf. gle1 morphants lose most zn-8 immunoreactivity, including the rostrally projecting nerves (asterisks in the control, A-C), but still retain the primary motoneurons (mnx1:GFP⁺) (D,F). Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bar: 50 μm.

Expression Data

Genes:	alcama GFP
Antibody:	zn-8
Fish:	ml2Tg ml2Tg + MO1-gle1 + MO2-gle1
Knockdown Reagents:	MO1-gle1 MO2-gle1
Anatomical Terms:	primary motor neuron axon primary motor neuron cell body secondary motor neuron axon secondary motor neuron cell body
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ml2Tg + MO1-gle1 + MO2-gle1
Knockdown Reagents:	MO1-gle1 MO2-gle1
Observed In:	secondary motor neuron spinal cord
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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