FIGURE

Fig. 1

ID

ZDB-FIG-091113-110

Publication

Sanek et al., 2009 - Zebrafish zic2a patterns the forebrain through modulation of Hedgehog-activated gene expression

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Fig. 1

Hh signaling activates six3b expression in the developing zebrafish forebrain. (A,B) At 15s, six3b is expressed normally in the anterior forebrain of vehicle-treated embryos (black arrowheads in A, 15/15 embryos) and dramatically reduced in cyclopamine-treated embryos (red arrowheads in B, 14/14 embryos treated from the 2- to 4-cell stage onwards). (C,D) At prim-10, ventral forebrain six3b expression is strong in wild-type (WT) embryos (black arrowheads in C, 67/92 embryos) and absent or greatly reduced in smo^-/- mutants (red arrowheads in D, 25/92 embryos). (E-H) Embryos injected with a low amount of shha mRNA (12.5 pg) show expanded six3b expression [green arrowheads in H, 10/23 embryos, 2 experiments (n=2)], but no change in ptc1 (F, 31/31 embryos, n=2). Embryos shown in panels A-H are lateral views. Panels A′-H′ are ventral views of the same embryos, anterior to the left.

Expression Data

Genes:	ptch2 six3b
Fish:	AB smo^b641/b641
Condition:	chemical treatment: Cyclopamine
Anatomical Terms:	central nervous system forebrain optic cup optic vesicle
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smo^b641/b641
Observed In:	smoothened signaling pathway
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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