Profile of catecholaminergic neurotransmitters, precursors, and degradation products in the brains of control and MPTP-treated adult zebrafish. Results are presented as percentage of the control values. Boxplot representation of the content of each neurochemical expressed as percentage of the control, with the boxes indicating the 25th and 75th percentiles and the whiskers the maximum and minimum values, showing all data. *** p < 0.001. Tyr: tyrosine; L-DOPA: levodopa; DA: dopamine; 3-MT: 3-Methoxytyramine; DOPAC: 3,4-Dihydroxyphenylacetic acid; NE: norepinephrine; NMN: normetanephrine.

Acute exposure to MPTP leads to hypokinesia in adult zebrafish. Fish were videorecorded at different times for 10 min in the open field test paradigm and the total distance travelled was calculated. Data are reported as mean ± SEM (n = 23–24 for 24 h after the second and the third injections, n = 12 for 48 h after the third injection, and n = 8–12 for 72 h after the third injection). * p < 0.05, ** p < 0.01, *** p < 0.001; Student’s t test.

Acute exposure to MPTP leads to a strong decrease in prepulse inhibition (PPI) percentage. The assay was performed using the platform Zebra_K, with 2 different interstimulus intervals between the prepulse and the pulse: 0.5 and 1.0 s. Data are presented as boxplots, where the box indicates the 25th and 75th percentiles, the thin line within the box marks the median, and the whiskers represent the maximum and minimum values, showing all data. ** p < 0.01; Mann–Whitney U test. Data are from 2 independent experiments with 4–5 adult wild-type short-fin zebrafish in each experimental group in each experiment.