FIGURE SUMMARY
Title

Investigation of Kelussia Odoratissima and Angelica Sinensis Similarities in Zebrafish-based In-vivo Bioactivity Assays and Their Chemical Composition

Authors
Rezaei, M., Fooladi, P., Norani, M., Crawford, A., Eisa-Beygi, S., Tahamtani, Y., Ayyari, M.
Source
Full text @ Galen med. j.
Fig. 1

GC chromatograms of essential oils of Kelussia odoratissima (i) and Angelica sinensis (ii); B: Structures of some of the main compounds (more than 5%) 1: n-Propylbenzene, 2: 2-Undecanone, 3: α-Copaene, 4: β-Thujaplicinol, 5: (Z)-γ-Bisabolene, 6: (3E)-Butylidene phthalide, 7: (Z)-Ligustilide, 8: (E)-Ligustilide, 9: Sclarene

Fig. 2

Schematics of zebrafish anti-angiogenesis and pBC regeneration bioassay methods. B: (1) Kelussia leave. (2) Angelica root. Bright-field and GFP filter view of: (3) 12 hpf Tg (fli: EGFP) embryo, (4) 3 dpf Tg(ins: GFP-NTR) larvae, (5) 4 dpf Tg(ins: GFP-NTR) larvae treated by MTZ, (6) 6 dpf Tg(ins: GFP-NTR) larvae, (7) 30 hpf Tg(fli: EGFP) embryo. Abbreviations: WT: Wild type; Tg: transgenic; dpf: days post fertilization; hpf: hours post fertilization; MTZ: metronidazole; PS: primary stock; WS: working stock; NT: Not treated; NC: Negative control; Ve: Vehicle; GFP: Green Fluorescent Protein NE: NECA.

Fig. 3

The anti-angiogenesis bioassay in the Tg(fli: EGFP) embryos A: Viability rate of zebrafish embryos exposed to different concentrations of extracts and essential oils. B: Relative vascular outgrowth in 30 hpf embryos after 18 h exposure to different samples in 7.81 µg/ml concentration (Larvae n:10). C: Vascular system of controls and treated zebrafish embryos. All the measurements were taken in triplicates and the values were presented as mean ± SD of three independent experiments, **P<0.01, ***P<0.001. KVL: Kelussia volatile oil leaves; KEL: Kelussia leaves; AER: Angelica root; AVR: Angelica volatile oil root.

Fig. 4

pBC regeneration assay in the Tg(ins: GFP-NTR) larvae. A: Viability rate of zebrafish larvae treated with different extractions. B: The average pBC area in 6 dpf larvae after regeneration in comparison with the control group. C: Fluorescent images of 6 dpf larvae show pBC mass, **P<0.01, ***P<0.001. KVL: Kelussia volatile oil leaves; KEL: Kelussia leaves; AER: Angelica root; AVR: Angelica volatile oil root; NC: Negative control.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Galen med. j.
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