Fig. 2 Schematics of zebrafish anti-angiogenesis and pBC regeneration bioassay methods. B: (1) Kelussia leave. (2) Angelica root. Bright-field and GFP filter view of: (3) 12 hpf Tg (fli: EGFP) embryo, (4) 3 dpf Tg(ins: GFP-NTR) larvae, (5) 4 dpf Tg(ins: GFP-NTR) larvae treated by MTZ, (6) 6 dpf Tg(ins: GFP-NTR) larvae, (7) 30 hpf Tg(fli: EGFP) embryo. Abbreviations: WT: Wild type; Tg: transgenic; dpf: days post fertilization; hpf: hours post fertilization; MTZ: metronidazole; PS: primary stock; WS: working stock; NT: Not treated; NC: Negative control; Ve: Vehicle; GFP: Green Fluorescent Protein NE: NECA.
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