PUBLICATION

Zebrafish tbx-c functions during formation of midline structures

Authors
Dheen, T., Sleptsova-Friedrich, I., Xu, Y., Clark, M., Lehrach, H., Gong, Z., and Korzh, V.
ID
ZDB-PUB-990525-5
Date
1999
Source
Development (Cambridge, England)   126(12): 2703-2713 (Journal)
Registered Authors
Clark, Matthew D., Dheen, S. Thameem, Gong, Zhiyuan, Korzh, Vladimir, Lehrach, Hans, Sleptsova-Freidrich, Inna, Xu, Yanfei
Keywords
T-box gene; notochord; somite; chordoneural hinge; motor neuron; overexpression; dominant-negative; myoD; shh; ntl; flh; islet1; tbx6; spt; animal cap
MeSH Terms
  • Amino Acid Sequence
  • Mesoderm/metabolism
  • Molecular Sequence Data
  • Motor Neurons/metabolism
  • Fetal Proteins/genetics
  • Fetal Proteins/metabolism
  • T-Box Domain Proteins*
  • Zebrafish Proteins*
  • Embryo, Nonmammalian
  • Embryology/methods
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism
  • Sequence Analysis
  • Gene Expression Regulation, Developmental
  • Nerve Tissue Proteins/genetics
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
(all 22)
PubMed
10331981 Full text @ Development
Abstract
Several genes containing the conserved T-box region in invertebrates and vertebrates have been reported recently. Here, we describe three novel members of the T-box gene family in zebrafish. One of these genes, tbx-c, is studied in detail. It is expressed in the axial mesoderm, notably, in the notochordal precursor cells immediately before formation of the notochord and in the chordoneural hinge of the tail bud, after the notochord is formed. In addition, its expression is detected in the ventral forebrain, sensory neurons, fin buds and excretory system. The expression pattern of tbx-c differs from that of the other two related genes, tbx-a and tbx-b. The developmental role of tbx-c has been analysed by overexpression of the full-length tbx-c mRNA and a truncated form of tbx-c mRNA, which encodes the dominant-negative Tbx-c. Overexpression of tbx-c causes expansion of the midline mesoderm and formation of ectopic midline structures at the expense of lateral mesodermal cells. In dominant-negative experiments, the midline mesoderm is reduced with the expansion of lateral mesoderm to the midline. These results suggest that tbx-c plays a role in formation of the midline mesoderm, particularly, the notochord. Moreover, modulation of tbx-c activity alters the development of primary motor neurons. Results of in vitro analysis in zebrafish animal caps suggest that tbx-c acts downstream of early mesodermal inducers (activin and ntl) and reveal an autoregulatory feedback loop between ntl and tbx-c. These data and analysis of midline (ntl-/- and flh-/-) and lateral mesoderm (spt-/-) mutants suggest that tbx-c may function during formation of the notochord.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
noton1/n1standard conditions1-4 somites
tbx16b104/b104standard conditions10-13 somites
1 - 2 of 2
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    b160
      		Indel
      n1
        		Small Deletion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        noton1/n1
        tbx16b104/b104
        tbxtab160/b160
        WT
        1 - 4 of 4
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Dheen, T., Sleptsova-Friedrich, I., Xu, Y., Clark, M., Lehrach, H., Gong, Z., and Korzh, V. (1999) Zebrafish tbx-c functions during formation of midline structures. Development (Cambridge, England). 126(12):2703-2713.