PUBLICATION

Expression of zebrafish bHLH genes ngn1 and nrd defines distinct stages of neural differentiation

Authors

Korzh, V., Sleptsova, I., Liao, J., He, J.Y., and Gong, Z.

ID

ZDB-PUB-980902-2

Date

1998

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 213: 92-104 (Journal)

Registered Authors

Gong, Zhiyuan, He, Jiangyan, Korzh, Vladimir, Liao, Ji

Keywords

neuronal bHLH; determination; differentiation; primary neurons; motoneurons; neural retina; pax-b; islet-1

MeSH Terms

Zebrafish/embryology*
Zebrafish/metabolism
Base Sequence
Amino Acid Sequence
Basic Helix-Loop-Helix Transcription Factors
Central Nervous System/embryology
Helix-Loop-Helix Motifs/genetics*
Homeodomain Proteins/genetics
DNA, Complementary
Sequence Homology, Amino Acid
LIM-Homeodomain Proteins
Zebrafish Proteins*
Transcription Factors*
Neurons
Nerve Tissue Proteins/genetics*
Molecular Sequence Data
Gene Expression Regulation, Developmental
Cloning, Molecular
Animals

(all 19)

PubMed

9733104 Full text @ Dev. Dyn.

Abstract

Two zebrafish bHLH genes, neurogenin-related gene I (ngn1) and neuroD (nrd), have been isolated. ngn1 expression is initiated at the end of gastrulation in the neural plate and defines broad domains of cells that probably possess an ability to develop as neurons. This finding suggests that ngn1 may play a role during determination of cell fate in neuroblasts. ngn1 and pax-b are expressed in a mutually exclusive manner. nrd expression follows that of ngn1 in restricted populations of cells selected from ngn1-positive clusters of cells. The earliest nrd-positive cells in the brain and the trunk are a subset of the primary neurons. ngn1 is not expressed in the eye. Here, nrd transcription is activated at 25 hours postfertilization in the ventral retina. Expression of islet-1 occurs in nrd-positive cells after expression of nrd, and the expression of the two genes partially overlaps in time. These observations suggest that during eye development nrd expression may follow expression of some other neurodetermination gene(s). This supports the idea that expression of nrd is a necessary step leading toward overt neuronal differentiation.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
isl1a	WT	standard conditions	5-9 somites	motor neuron	ISH	Fig. 6
isl1a	WT	standard conditions	Bud	neural plate	ISH	Fig. 6
neurod1	WT	standard conditions	14-19 somites	diencephalon	ISH	Fig. 6
neurod1	WT	standard conditions	Bud	forebrain neural plate	ISH	Fig. 6
neurod1	WT	standard conditions	Prim-5	hindbrain neural tube	ISH	Fig. 7
neurod1	WT	standard conditions	14-19 somites	lateral line system	ISH	Fig. 6
neurod1	WT	standard conditions	5-9 somites	motor neuron	ISH	Fig. 6
neurod1	WT	standard conditions	14-19 somites	motor neuron	ISH	Fig. 6
neurod1	WT	standard conditions	5-9 somites	neural plate	ISH	Fig. 6
neurod1	WT	standard conditions	Bud	neural plate	ISH	Fig. 6

1 - 10 of 44

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Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-isl	monoclonal	isl1a isl2a	IgG1	Mouse

Orthology

Engineered Foreign Genes

Mapping

Korzh et al., 1998 ZDB-PUB-980902-2 PMID:9733104

Expression of zebrafish bHLH genes ngn1 and nrd defines distinct stages of neural differentiation

Korzh et al., 1998

ZDB-PUB-980902-2
PMID:9733104