PUBLICATION

Functional role for Taz during hindbrain ventricle morphogenesis

Authors
Dicipulo, R., Selland, L.G., Carpenter, R.G., Waskiewicz, A.J.
ID
ZDB-PUB-250315-3
Date
2025
Source
PLoS One   20: e0313262e0313262 (Journal)
Registered Authors
Waskiewicz, Andrew
Keywords
none
MeSH Terms
  • Serine-Threonine Kinase 3
  • Transcriptional Coactivator with PDZ-Binding Motif Proteins
  • Gene Expression Regulation, Developmental
  • Signal Transduction
  • Zebrafish*/embryology
  • Cell Polarity
  • Animals
  • Cerebral Ventricles/embryology
  • Cerebral Ventricles/metabolism
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Actins/metabolism
  • Rhombencephalon*/embryology
  • Rhombencephalon*/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Morphogenesis*
(all 17)
PubMed
40080483 Full text @ PLoS One
Abstract
The brain ventricle system, composed of the ventricular cavities and the cerebral spinal fluid within, performs critical functions including circulation of nutrients, removal of wastes, and cushioning of neural tissues. Development of the hindbrain ventricle requires a series of factors that coordinate its initial formation and subsequent inflation. Previous work has demonstrated that the transcriptional co-activator Taz (also known as WW domain-containing transcription regulator protein 1, Wwtr1), a component of Hippo signalling, is active at hindbrain rhombomere boundaries where it is regulated by mechanotransduction and promotes proliferation. Here, we demonstrate that Taz is also a critical regulator of hindbrain ventricle development. Zebrafish embryos that lack Taz protein fail to undergo initial midline separation of the hindbrain ventricle. Furthermore, the ventricle phenotype is a result of disorganized cytoskeletal F-actin and apicobasal polarity components. In addition, we have demonstrated that the hindbrain rhombomere boundaries are a location of active Wnt-Hippo crosstalk. Through our work, we propose a model where Taz protein is stabilized at rhombomere boundaries and promotes proper cell polarity necessary for formation of the brain ventricle.
Genes / Markers
Figures
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
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Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
yap1CRISPR15-yap1CRISPR
1 - 1 of 1
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Fish
No data available
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-wwtr1monoclonalIgGRabbit
Ab1-yap1polyclonalRabbit
zs-4monoclonalIgG1Mouse
1 - 3 of 3
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Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
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