PUBLICATION
Ccn2a acts downstream of cx43 to influence joint formation during zebrafish fin regeneration
- Authors
- Hyland, V., Iovine, M.K.
- ID
- ZDB-PUB-250218-9
- Date
- 2025
- Source
- Biology Open : (Journal)
- Registered Authors
- Iovine, M. Kathryn
- Keywords
- Cellular communication network factor 2, Connexin43, Joint formation, Skeletal regeneration, Yap, Zebrafish, β-catenin
- MeSH Terms
-
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- beta Catenin/metabolism
- Animals
- Gene Expression Regulation
- Regeneration*
- Connexin 43*/genetics
- Connexin 43*/metabolism
- Zebrafish*
- Signal Transduction
- Joints/embryology
- Joints/metabolism
- Animal Fins*/metabolism
- Animal Fins*/physiology
- PubMed
- 39963716 Full text @ Biol. Open
Citation
Hyland, V., Iovine, M.K. (2025) Ccn2a acts downstream of cx43 to influence joint formation during zebrafish fin regeneration. Biology Open. :.
Abstract
This study provides new insights into the molecular pathways dictating skeletal patterning during zebrafish fin regeneration. Connexin43 (Cx43) is known to influence skeletal patterning by inhibiting evx1 expression and thereby regulating the timing of joint formation. Here, we add to this pathway by demonstrating that cellular communication network factor 2 (ccn2a) also contributes to this pathway. We find that Ccn2a appears to act downstream of Cx43 and similarly inhibits joint formation by inhibiting evx1 expression. Pharmacological inhibition of β-catenin demonstrates that ccn2a is likely regulated by β-catenin. Additionally, this paper provides evidence that Yap signaling also contributes to joint formation through regulating ccn2a. These findings provide novel insights into the role of Ccn2a during skeletal patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping