PUBLICATION

Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis

Authors
Pan, Y.J., Tong, S.K., Hsu, C.W., Weng, J.H., Chung, B.C.
ID
ZDB-PUB-220422-6
Date
2022
Source
Frontiers in cell and developmental biology   10: 866267 (Journal)
Registered Authors
Chung, Bon-chu
Keywords
development, gonad, meiosis, oocyte, ovary, sex, sycp3, transcriptome
MeSH Terms
none
PubMed
35445010 Full text @ Front Cell Dev Biol
Abstract
Zebrafish is a popular research model; but its mechanism of sex determination is unclear and the sex of juvenile fish cannot be distinguished. To obtain fish with defined sex, we crossed domesticated zebrafish with the Nadia strain that has a female-dominant W segment. These fish were placed on a ziwi:GFP background to facilitate sorting of fluorescent germ cells for transcriptomic analysis. We analyzed the transcriptomes of germ cells at 10-14 days postfertilization (dpf), when sex dimorphic changes started to appear. Gene ontology showed that genes upregulated in the 10-dpf presumptive females are involved in cell cycles. This correlates with our detection of increased germ cell numbers and proliferation. We also detected upregulation of meiotic genes in the presumptive females at 14 dpf. Disruption of a meiotic gene, sycp3, resulted in sex reversal to infertile males. The germ cells of sycp3 mutants could not reach diplotene and underwent apoptosis. Preventing apoptosis by disrupting tp53 restored female characteristics in sycp3 mutants, demonstrating that adequate germ cells are required for female development. Thus, our transcriptome and gene mutation demonstrate that initial germ cell proliferation followed by meiosis is the hallmark of female differentiation in zebrafish.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
as5d
    		Small Deletion
    as8d
      		Small Deletion
      uc1TgTransgenic Insertion
        zdf1
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          sycp3CRISPR1-sycp3CRISPR
          1 - 1 of 1
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          Fish
          No data available
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab2-pcnamonoclonalIgG2aMouse
          Ab2-sycp3polyclonal
            		IgGRabbit
            Ab3-rad51polyclonalRabbit
            Ab4-ddx4polyclonalIgGRabbit
            Ab5-casp3polyclonal
              		Rabbit
              1 - 5 of 5
              Show
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              1 - 1 of 1
              Show
              Mapping
              No data available
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              Citation
              Pan, Y.J., Tong, S.K., Hsu, C.W., Weng, J.H., Chung, B.C. (2022) Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis. Frontiers in cell and developmental biology. 10:866267.