PUBLICATION

Extracellular mechanical forces drive endocardial cell volume decrease during zebrafish cardiac valve morphogenesis

Authors
Vignes, H., Vagena-Pantoula, C., Prakash, M., Fukui, H., Norden, C., Mochizuki, N., Jug, F., Vermot, J.
ID
ZDB-PUB-220305-15
Date
2022
Source
Developmental Cell   57(5): 598-609.e5 (Journal)
Registered Authors
Fukui, Hajime, Mochizuki, Naoki, Norden, Caren, Vermot, Julien
Keywords
ECM, actomyosin, cell polarity, endocardium, mechanobiology, mechanotransduction, notch, shear stress, zebrafish
MeSH Terms
  • Mechanotransduction, Cellular
  • TRPV Cation Channels/metabolism
  • Morphogenesis
  • Zebrafish*/metabolism
  • Cell Size
  • Zebrafish Proteins*/metabolism
  • Animals
  • Heart Valves/metabolism
(all 8)
PubMed
35245444 Full text @ Dev. Cell
Abstract
Organ morphogenesis involves dynamic changes of tissue properties while cells adapt to their mechanical environment through mechanosensitive pathways. How mechanical cues influence cell behaviors during morphogenesis remains unclear. Here, we studied the formation of the zebrafish atrioventricular canal (AVC) where cardiac valves develop. We show that the AVC forms within a zone of tissue convergence associated with the increased activation of the actomyosin meshwork and cell-orientation changes. We demonstrate that tissue convergence occurs with a reduction of cell volume triggered by mechanical forces and the mechanosensitive channel TRPP2/TRPV4. Finally, we show that the extracellular matrix component hyaluronic acid controls cell volume changes. Together, our data suggest that multiple force-sensitive signaling pathways converge to modulate cell volume. We conclude that cell volume reduction is a key cellular feature activated by mechanotransduction during cardiovascular morphogenesis. This work further identifies how mechanical forces and extracellular matrix influence tissue remodeling in developing organs.
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Marker Marker Type Name
DsRedxEFGDsRedx
EGFPEFGEGFP
GAL4FFEFGGAL4FF
KaedeEFGKaede
mCherryEFGmCherry
TENSEFGTENS
TFPEFGTFP
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