PUBLICATION

Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism

Authors
Pulgar, E., Schwayer, C., Guerrero, N., López, L., Márquez, S., Härtel, S., Soto, R., Heisenberg, C.P., Concha, M.
ID
ZDB-PUB-210828-43
Date
2021
Source
eLIFE   10: (Journal)
Registered Authors
Concha, Miguel, Heisenberg, Carl-Philipp
Keywords
developmental biology, zebrafish
MeSH Terms
  • Epithelial Cells/physiology*
  • Time Factors
  • Cell Differentiation*
  • Cell Communication*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Cell Lineage
  • Morphogenesis
  • Gene Expression Regulation, Developmental
  • Embryo, Nonmammalian/physiology
  • Cell Movement*
  • Stem Cells/physiology*
  • Animals
  • Cell Adhesion
  • Animals, Genetically Modified
(all 15)
PubMed
34448451 Full text @ Elife
Abstract
The developmental strategies used by progenitor cells to allow a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. Here we uncovered a mechanism of progenitor cell allocation that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. Progenitors of the zebrafish laterality organ originate from the superficial epithelial enveloping layer by an apical constriction process of cell delamination. During this process, progenitors retain long-lasting apical contacts that enable the epithelial layer to pull a subset of progenitors on their way to the vegetal pole. The remaining delaminated cells follow the movement of apically attached progenitors by a protrusion-dependent cell-cell contact mechanism, avoiding sequestration by the adjacent endoderm, ensuring their collective fate and allocation at the site of differentiation. Thus, we reveal that incomplete delamination serves as a cellular platform for coordinated tissue movements during development.
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e119TgTransgenic Insertion
    e2212TgTransgenic Insertion
      s870TgTransgenic Insertion
        s944TgTransgenic Insertion
          vu119TgTransgenic Insertion
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            Target Reagent Reagent Type
            cdh1MO4-cdh1MRPHLNO
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            Orthology
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            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            mCherryEFGmCherry
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            Citation
            Pulgar, E., Schwayer, C., Guerrero, N., López, L., Márquez, S., Härtel, S., Soto, R., Heisenberg, C.P., Concha, M. (2021) Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. eLIFE. 10:.