PUBLICATION

RNA-induced inflammation and migration of precursor neurons initiates neuronal circuit regeneration in zebrafish

Authors
Vandestadt, C., Vanwalleghem, G.C., Khabooshan, M.A., Douek, A.M., Castillo, H.A., Li, M., Schulze, K., Don, E., Stamatis, S.A., Ratnadiwakara, M., Änkö, M.L., Scott, E.K., Kaslin, J.
ID
ZDB-PUB-210825-6
Date
2021
Source
Developmental Cell   56: 2364-2380.e8 (Journal)
Registered Authors
Don, Emily, Douek, Alon M., Kaslin, Jan, Li, Mei, Scott, Ethan
Keywords
SRC, TLR3, adult neurogenesis, dsRNA, epimorphic, innate immunity, morphallactic, neural stem cell, repair, spinal cord
MeSH Terms
  • RNA/metabolism*
  • Nerve Regeneration*
  • Cell Movement*
  • Neural Stem Cells/metabolism*
  • Neural Stem Cells/physiology
  • Animals
  • Zebrafish
(all 7)
PubMed
34428400 Full text @ Dev. Cell
Abstract
Tissue regeneration and functional restoration after injury are considered as stem- and progenitor-cell-driven processes. In the central nervous system, stem cell-driven repair is slow and problematic because function needs to be restored rapidly for vital tasks. In highly regenerative vertebrates, such as zebrafish, functional recovery is rapid, suggesting a capability for fast cell production and functional integration. Surprisingly, we found that migration of dormant "precursor neurons" to the injury site pioneers functional circuit regeneration after spinal cord injury and controls the subsequent stem-cell-driven repair response. Thus, the precursor neurons make do before the stem cells make new. Furthermore, RNA released from the dying or damaged cells at the site of injury acts as a signal to attract precursor neurons for repair. Taken together, our data demonstrate an unanticipated role of neuronal migration and RNA as drivers of neural repair.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a131TgTransgenic Insertion
    i114TgTransgenic Insertion
      i131TgTransgenic Insertion
        ia6TgTransgenic Insertion
          jf5TgTransgenic Insertion
            knu3TgTransgenic Insertion
              mi2001TgTransgenic Insertion
                mnu302TgTransgenic Insertion
                  mnu304TgTransgenic Insertion
                    mq8TgTransgenic Insertion
                      1 - 10 of 17
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      tlr3CRISPR1-tlr3CRISPR
                      tlr3CRISPR2-tlr3CRISPR
                      tlr22CRISPR1-tlr22CRISPR
                      tlr22CRISPR2-tlr22CRISPR
                      1 - 4 of 4
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                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CeruleanEFGCerulean
                      CreEFGCre
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GCaMPEFGGCaMP
                      GFPEFGGFP
                      mCherryEFGmCherry
                      TagBFPEFGTagBFP
                      TomatoEFGTomato
                      VenusEFGVenus
                      1 - 10 of 11
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                      Mapping
                      No data available