PUBLICATION

H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish

Authors
Jiang, W., Liu, C., Deng, M., Wang, F., Ren, X., Fan, Y., Du, J., Wang, Y.
ID
ZDB-PUB-201130-5
Date
2020
Source
Stroke and vascular neurology   6(2): 244-251 (Journal)
Registered Authors
Du, Jiu Lin
Keywords
brain, stroke, vascular malformation
MeSH Terms
  • Animals
  • Hydrogen Sulfide*/metabolism
  • Cystathionine gamma-Lyase/genetics
  • Cystathionine gamma-Lyase/metabolism
  • Zebrafish*/metabolism
  • Endothelial Cells/metabolism
  • Brain/metabolism
  • Cystathionine beta-Synthase/genetics
  • Cystathionine beta-Synthase/metabolism
(all 9)
PubMed
33246971 Full text @ Stroke Vasc Neurol
Abstract
Background Hydrogen sulphide (H2S) is considered as the third member of the gasotransmitter family, along with nitric oxide (NO) and carbon monoxide. H2S has been reported to induce angiogenesis by promoting the growth, migration and tube-like structure formation of endothelial cells. Those studies were conducted in conditions of cell culture, mouse Matrigel plug assay model, rat wound healing model or rat hindlimb ischaemia model. Recent in vivo studies showed the physiological importance of H2S in muscle angiogenesis. However, the importance of endogenous H2S for brain angiogenesis during development remains unknown. We therefore aimed at determining the role of H2S in brain vascular development.
Methods and results Both knockdown and knockout of H2S-producing enzymes, cystathionine β-synthase (cbs) and cystathionine γ-lyase (cth), using morpholino oligonucleotides and clustered regularly interspaced short palindromic repeats/Cas9-mediated mutation, impaired brain vascular development of larval zebrafish. Incubation with the slow-releasing H2S donor GYY4137 alleviated the defects of brain vascular development in cbs and cth morphants. Quantitative analysis of the midbrain vascular network showed that H2S enhances angiogenesis without affecting the topological structure of the brain vasculature. Mechanically, nitric oxide synthase 2a (nos2a) expression and NO production were decreased in both cbs and cth morphants. Overexpression of nos2a by coinjection of cbs or cth MO with full-length zebrafish nos2a mRNA alleviated the brain vascular developmental defects in cbs and cth morphants.
Conclusion We conclude that H2S promotes brain developmental angiogenesis via the NOS/NO pathway in zebrafish.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s843TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    cbsbCRISPR2-cbsbCRISPR
    cbsbMO5-cbsbMRPHLNO
    cthCRISPR1-cthCRISPR
    cthMO2-cthMRPHLNO
    1 - 4 of 4
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    Fish
    No data available
    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab1-cbspolyclonal
      		Rabbit
      Ab1-vegfmonoclonal
        		IgG2bMouse
        Ab2-cthmonoclonal
          		IgG1Mouse
          Ab9-mapkmonoclonal
            		IgGRabbit
            Ab23-mapkmonoclonal
              		IgG1Mouse
              1 - 5 of 5
              Show
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              1 - 1 of 1
              Show
              Mapping
              No data available
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              Citation
              Jiang, W., Liu, C., Deng, M., Wang, F., Ren, X., Fan, Y., Du, J., Wang, Y. (2020) H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish. Stroke and vascular neurology. 6(2):244-251.