PUBLICATION

Morphogenesis is transcriptionally coupled to neurogenesis during peripheral olfactory organ development

Authors
Aguillon, R., Madelaine, R., Aguirrebengoa, M., Guturu, H., Link, S., Dufourcq, P., Lecaudey, V., Bejerano, G., Blader, P., Batut, J.
ID
ZDB-PUB-201120-23
Date
2020
Source
Development (Cambridge, England)   147(24): (Journal)
Registered Authors
Batut, Julie, Blader, Patrick, Dufourcq, Pascale, Lecaudey, Virginie, Madelaine, Romain
Keywords
Chemokine, Morphogenesis, Neurogenesis, Neurogenin1, Olfactory placode, Zebrafish
MeSH Terms
  • Transcription Initiation Site
  • Olfactory Mucosa/growth & development*
  • Embryonic Development/genetics
  • Nerve Tissue Proteins/genetics*
  • Neurons/metabolism
  • Embryo, Nonmammalian
  • Neurogenesis/genetics*
  • Animals
  • E-Box Elements/genetics
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Receptors, CXCR4/genetics*
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • Morphogenesis/genetics*
  • Mutation/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Zebrafish Proteins/genetics*
(all 17)
PubMed
33144399 Full text @ Development
Abstract
Sense organs acquire their distinctive shapes concomitantly with the differentiation of sensory cells and neurons necessary for their function. While our understanding of the mechanisms controlling morphogenesis and neurogenesis in these structures has grown, how these processes are coordinated remains largely unexplored. Neurogenesis in the zebrafish olfactory epithelium requires the bHLH proneural transcription factor Neurogenin1 (Neurog1). To address whether Neurog1 also controls morphogenesis, we analysed the migratory behaviour of early olfactory neural progenitors in neurog1 mutant embryos. Our results indicate that the oriented movements of these progenitors are disrupted in this context. Morphogenesis is similarly affected by mutations in the chemokine receptor gene, cxcr4b, suggesting it is a potential Neurog1 target gene. We find that Neurog1 directly regulates cxcr4b through an E-boxes cluster located just upstream of the cxcr4b transcription start site. Our results suggest that proneural transcription factors, such as Neurog1, directly couple distinct aspects of nervous system development.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fu10TgTransgenic Insertion
    hi1059TgTransgenic Insertion
    sb1TgTransgenic Insertion
      t26035
        		Point Mutation
        t30516
          		Point Mutation
          ups10TgTransgenic Insertion
            1 - 6 of 6
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            cxcr4bCRISPR3-cxcr4bCRISPR
            cxcr4bCRISPR4-cxcr4bCRISPR
            1 - 2 of 2
            Show
            Fish
            No data available
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            mCherryEFGmCherry
            1 - 3 of 3
            Show
            Mapping
            No data available
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            Citation
            Aguillon, R., Madelaine, R., Aguirrebengoa, M., Guturu, H., Link, S., Dufourcq, P., Lecaudey, V., Bejerano, G., Blader, P., Batut, J. (2020) Morphogenesis is transcriptionally coupled to neurogenesis during peripheral olfactory organ development. Development (Cambridge, England). 147(24):.