PUBLICATION

Greb1 is required for axial elongation and segmentation in vertebrate embryos

Authors

Prajapati, R.S., Mitter, R., Vezzaro, A., Ish-Horowicz, D.

ID

ZDB-PUB-200129-6

Date

2020

Source

Biology Open 9(2): (Journal)

Registered Authors

Keywords

Axial stem cells, Clock, Neural tube, Progenitors, Somites, Tailbud, Transcriptome

MeSH Terms

Computational Biology/methods
Stem Cells/cytology
Stem Cells/metabolism
Mice
Embryonic Development/genetics*
Gene Expression Profiling
Zebrafish Proteins/genetics*
Gene Knockdown Techniques
Neoplasm Proteins/genetics*
Phenotype
Animals
Membrane Proteins/genetics*
Morphogenesis/genetics*
Transcriptome
Gene Expression Regulation, Developmental

(all 15)

PubMed

31988092 Full text @ Biol. Open

Abstract

During vertebrate embryonic development, the formation of axial structures is driven by a population of stem-like cells that reside in a region of the tailbud called the chordoneural hinge (CNH). We have compared the mouse CNH transcriptome with those of surrounding tissues and shown that the CNH and tailbud mesoderm are transcriptionally similar, and distinct from the presomitic mesoderm. Amongst CNH-enriched genes are several that are required for axial elongation, including Wnt3a, Cdx2, Brachyury/T and Fgf8, and androgen/estrogen receptor nuclear signalling components such as Greb1 We show that the pattern and duration of tailbud Greb1 expression is conserved in mouse, zebrafish, and chicken embryos, and that Greb1 is required for axial elongation and somitogenesis in zebrafish embryos. The axial truncation phenotype of Greb1 morphant embryos can be explained by much reduced expression of No tail (Ntl/Brachyury) which is required for axial progenitor maintenance. Posterior segmentation defects in the morphants (including misexpression of genes such as mespb, myoD and papC) appear to result, in part, from lost expression of the segmentation clock gene, her7.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-greb1	standard conditions	Prim-5	somite myod1 expression increased distribution, abnormal	Fig. 3.
WT + MO1-greb1	standard conditions	Prim-5	somite myod1 expression spatial pattern, abnormal	Fig. 3.
WT + MO1-greb1	standard conditions	Prim-5	somite morphology, abnormal	Fig. 3.
WT + MO1-greb1	standard conditions	Prim-5	vertical myoseptum xirp2a expression decreased amount, abnormal	Fig. 3.
WT + MO1-greb1	standard conditions	Prim-5	vertical myoseptum xirp2a expression spatial pattern, abnormal	Fig. 3.
WT + MO2-greb1	standard conditions	10-13 somites	segmental plate her7 expression absent, abnormal	Fig. 3.
WT + MO2-greb1	standard conditions	10-13 somites	somite mespba expression decreased amount, abnormal	Fig. 3.
WT + MO2-greb1	standard conditions	10-13 somites	somite pcdh8 expression decreased amount, abnormal	Fig. 3.
WT + MO2-greb1	standard conditions	10-13 somites	tail bud her7 expression absent, abnormal	Fig. 3.
WT + MO2-greb1	standard conditions	10-13 somites	tail bud tbxta expression decreased amount, abnormal	Fig. 3.

1 - 10 of 15

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
greb1	MO1-greb1	MRPHLNO
greb1	MO2-greb1	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
WT
WT + MO1-greb1
WT + MO2-greb1

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Prajapati et al., 2020 ZDB-PUB-200129-6 PMID:31988092

Greb1 is required for axial elongation and segmentation in vertebrate embryos

Prajapati et al., 2020

ZDB-PUB-200129-6
PMID:31988092